Article(id=1292126420763959567, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1292126409196064779, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2026.02.007, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1765900800000, receivedDateStr=2025-12-17, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785996545258, onlineDateStr=2026-08-06, pubDate=1769529600000, pubDateStr=2026-01-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785996545258, onlineIssueDateStr=2026-08-06, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785996545258, creator=13701087609, updateTime=1785996545258, updator=13701087609, issue=Issue{id=1292126409196064779, tenantId=1146029695717560320, journalId=1246415772164075586, year='2026', volume='42', issue='2', pageStart='151', pageEnd='300', issueExtLink='null', onlineDate='null', pubDate='1769529600000', pubDateStr='2026-01-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785996542500, creator='13701087609', updateTime=1786014671444, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1292202447569707674, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1292126409196064779, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1292202447569707675, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1292126409196064779, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=192, endPage=196, ext={EN=ArticleExt(id=1292126420973674768, articleId=1292126420763959567, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Investigation of phenotypic and genotypic correlations of the vanM gene cluster in Enterococcus faecium, columnId=1246531407326105792, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Clinical and Basic Bridging Research, runingTitle=null, highlight=null, articleAbstract=
Objective

To reveal the association between the vanM gene and different glycopeptide resistance phenotypes in Enterococcus faecium, drug susceptibility testing and sequencing analysis were conducted on four E. faecium strains carrying the vanM gene.

Methods

The broth microdilution method was employed to determine the susceptibility of four E. faecium strains to glycopeptide antibiotics. Polymerase chain reaction (PCR) was performed to detect the vancomycin-resistant genotypes, while population analysis profiling (PAP) was used to identify the presence of heteroresistant strains among vancomycin-susceptible isolates. Quantitative real-time PCR (qRT-PCR) was conducted to analyze the relative expression level of the vanM gene in E. faecium, and genomic sequencing was carried out for alignment of the resistance gene cluster.

Results

All four E. faecium strains carried the vanM gene. Strain SZD1 was susceptible to vancomycin, whereas SZD2 was identified as a vancomycin-heteroresistant strain. Strain SZD3 exhibited a high relative expression level of the vanM gene and showed resistance to vancomycin. In addition, strain SZD4 harbored both vanA and vanM genotypes, conferring high-level resistance to vancomycin.

Conclusion

E. faecium strains harboring the vanM gene can exhibit diverse glycopeptide resistance phenotypes, and such variations may be associated with the structure, copy number, and expression level of the vanM gene cluster, as well as the coexistence of other resistance genes.

, authors=Bo SU1, Xiao-lin CHEN2, Bo ZHENG1, authorsList=Bo SU, Xiao-lin CHEN, Bo ZHENG, authorCompany=null, correspAuthors=Bo ZHENG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1292126421233721617, articleId=1292126420763959567, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=屎肠球菌中vanM基因簇表型与基因型相关性研究, columnId=1246531407485489349, journalTitle=中国临床药理学杂志, columnName=临床与基础桥接研究, runingTitle=null, highlight=null, articleAbstract=
目的

对4株携带vanM基因的屎肠球菌进行药物敏感性试验和测序分析,从而揭示屎肠球菌中vanM基因与不同糖肽类耐药表型的关联。

方法

用微量肉汤稀释法对4株屎肠球菌进行糖肽类抗菌药物敏感性试验,PCR检测万古霉素耐药基因型,菌群谱型分析(PAP)检测万古霉素敏感菌株中是否存在异质性耐药菌株。用qRT-PCR分析屎肠球菌中vanM基因的相对表达水平,基因组测序比对耐药基因簇。

结果

4株屎肠球菌均携带vanM基因,SZD1对万古霉素敏感,SZD2为万古霉素异质性耐药菌株,SZD3中vanM基因相对表达量高,对万古霉素耐药,SZD4携带vanAvanM双基因型,对万古霉素高度耐药。

结论

携带vanM基因的屎肠球菌可呈现出不同的糖肽类耐药表型,该差异可能与vanM基因簇的结构、拷贝数、表达水平以及是否与其他耐药基因共存等相关。

, authors=苏博1, 陈晓林2, 郑波1, authorsList=苏博, 陈晓林, 郑波, authorCompany=null, correspAuthors=郑波, authorNote=

苏博(1999-),男,硕士研究生,主要从事细菌耐药机制方面的研究

, correspAuthorsNote=
郑波,教授,主任医师,博士生导师 Tel: (010)82802542 E-mail:
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苏博(1999-),男,硕士研究生,主要从事细菌耐药机制方面的研究

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苏博(1999-),男,硕士研究生,主要从事细菌耐药机制方面的研究

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屎肠球菌中vanM基因簇表型与基因型相关性研究
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苏博 1 , 陈晓林 2 , 郑波 1
中国临床药理学杂志 | 临床与基础桥接研究 2026,42(2): 192-196
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中国临床药理学杂志 |临床与基础桥接研究 2026 , 42 (2) : 192 -196
屎肠球菌中vanM基因簇表型与基因型相关性研究
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苏博1, 陈晓林2, 郑波1
作者信息
  • 1.北京大学 第一医院 临床药理研究所,北京 100034
  • 2.北京大学 第一医院 泌尿外科,北京 100034
通讯作者:
郑波,教授,主任医师,博士生导师 Tel: (010)82802542 E-mail:
作者简介:

苏博(1999-),男,硕士研究生,主要从事细菌耐药机制方面的研究

Investigation of phenotypic and genotypic correlations of the vanM gene cluster in Enterococcus faecium
Bo SU1, Xiao-lin CHEN2, Bo ZHENG1
Affiliations
  • 1.Institute of Clinical Pharmacology, Peking University First Hospital, Beijing 100034, China
  • 2.Department of Urology, Peking University First Hospital, Beijing 100034, China
出版时间: 2026-01-28 doi: 10.13699/j.cnki.1001-6821.2026.02.007
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目的

对4株携带vanM基因的屎肠球菌进行药物敏感性试验和测序分析,从而揭示屎肠球菌中vanM基因与不同糖肽类耐药表型的关联。

方法

用微量肉汤稀释法对4株屎肠球菌进行糖肽类抗菌药物敏感性试验,PCR检测万古霉素耐药基因型,菌群谱型分析(PAP)检测万古霉素敏感菌株中是否存在异质性耐药菌株。用qRT-PCR分析屎肠球菌中vanM基因的相对表达水平,基因组测序比对耐药基因簇。

结果

4株屎肠球菌均携带vanM基因,SZD1对万古霉素敏感,SZD2为万古霉素异质性耐药菌株,SZD3中vanM基因相对表达量高,对万古霉素耐药,SZD4携带vanAvanM双基因型,对万古霉素高度耐药。

结论

携带vanM基因的屎肠球菌可呈现出不同的糖肽类耐药表型,该差异可能与vanM基因簇的结构、拷贝数、表达水平以及是否与其他耐药基因共存等相关。

万古霉素  /  替考拉宁  /  屎肠球菌  /  vanM基因簇
Objective

To reveal the association between the vanM gene and different glycopeptide resistance phenotypes in Enterococcus faecium, drug susceptibility testing and sequencing analysis were conducted on four E. faecium strains carrying the vanM gene.

Methods

The broth microdilution method was employed to determine the susceptibility of four E. faecium strains to glycopeptide antibiotics. Polymerase chain reaction (PCR) was performed to detect the vancomycin-resistant genotypes, while population analysis profiling (PAP) was used to identify the presence of heteroresistant strains among vancomycin-susceptible isolates. Quantitative real-time PCR (qRT-PCR) was conducted to analyze the relative expression level of the vanM gene in E. faecium, and genomic sequencing was carried out for alignment of the resistance gene cluster.

Results

All four E. faecium strains carried the vanM gene. Strain SZD1 was susceptible to vancomycin, whereas SZD2 was identified as a vancomycin-heteroresistant strain. Strain SZD3 exhibited a high relative expression level of the vanM gene and showed resistance to vancomycin. In addition, strain SZD4 harbored both vanA and vanM genotypes, conferring high-level resistance to vancomycin.

Conclusion

E. faecium strains harboring the vanM gene can exhibit diverse glycopeptide resistance phenotypes, and such variations may be associated with the structure, copy number, and expression level of the vanM gene cluster, as well as the coexistence of other resistance genes.

vancomycin  /  teicoplanin  /  Enterococcus faecium  /  vanM gene cluster
苏博, 陈晓林, 郑波. 屎肠球菌中vanM基因簇表型与基因型相关性研究. 中国临床药理学杂志, 2026 , 42 (2) : 192 -196 . DOI: 10.13699/j.cnki.1001-6821.2026.02.007
Bo SU, Xiao-lin CHEN, Bo ZHENG. Investigation of phenotypic and genotypic correlations of the vanM gene cluster in Enterococcus faecium[J]. Chinese Journal of Clinical Pharmacology, 2026 , 42 (2) : 192 -196 . DOI: 10.13699/j.cnki.1001-6821.2026.02.007

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doi: 10.13699/j.cnki.1001-6821.2026.02.007
  • 接收时间:2025-12-17
  • 首发时间:2026-08-06
  • 出版时间:2026-01-28
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  • 收稿日期:2025-12-17
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    1.北京大学 第一医院 临床药理研究所,北京 100034
    2.北京大学 第一医院 泌尿外科,北京 100034

通讯作者:

郑波,教授,主任医师,博士生导师 Tel: (010)82802542 E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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