中国临床药理学杂志

穿心莲内酯调节miR-218-5p/SOX5影响口腔鳞状细胞癌细胞上皮间质转化的研究

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张芳英 ^a , 黄新帮 ^b , 黄迪 ^c , 曾以旺 ^a , 黄根生 ^c

中国临床药理学杂志 | 临床与基础桥接研究 2025,41(19): 2767-2773

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中国临床药理学杂志 |临床与基础桥接研究 2025 , 41 (19) : 2767 -2773

穿心莲内酯调节miR-218-5p/SOX5影响口腔鳞状细胞癌细胞上皮间质转化的研究

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张芳英^a, 黄新帮^b, 黄迪^c, 曾以旺^a, 黄根生^c

作者信息

^a.赣州市人民医院，药剂科，江西赣州 341000

^b.赣州市人民医院，口腔颌面外科，江西赣州 341000

^c.赣州市人民医院，口腔科，江西赣州 341000

通讯作者:

黄根生，副主任医师 MP: 15879748676 E-mail: zfy50062@163.com

作者简介:

张芳英（1988-），女，主管药师，主要从事临床药学、基础医学方面的工作和研究

Research of andrographolide modulates epithelial-mesenchymal transition in oral squamous cell carcinoma by miR-218-5p/SOX5 pathways

Fang-ying ZHANG^a, Xin-bang HUANG^b, Di HUANG^c, Yi-wang ZENG^a, Gen-sheng HUANG^c

Affiliations

^a.Department of Pharmacy, Ganzhou People’s Hospital, Ganzhou 341000, Jiangxi Province, China

^b.Department of Maxillofacial Surgery, Ganzhou People’s Hospital, Ganzhou 341000, Jiangxi Province, China

^c.Department of Stomatology, Ganzhou People’s Hospital, Ganzhou 341000, Jiangxi Province, China

出版时间: 2025-10-17 doi: 10.13699/j.cnki.1001-6821.2025.19.012

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摘要

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目的

探讨穿心莲内酯调节微小RNA（miR）-218-5p/SRY-box转录因子5（SOX5）对口腔鳞状细胞癌细胞增殖、侵袭和上皮间质转化（EMT）的影响。

方法

将CAL-27细胞分为空白组（常规培养）、穿心莲内酯组（15 μmol·L^-1穿心莲内酯处理）、穿心莲内酯+inhibitor NC组（15 μmol·L^-1穿心莲内酯+转染inhibitor NC处理）、穿心莲内酯+miR-218-5p inhibitor组（15 μmol·L^-1穿心莲内酯+转染miR-218-5p inhibitor处理）、穿心莲内酯+miR-218-5p inhibitor+si-NC组（15 μmol·L^-1穿心莲内酯+共转染miR-218-5p inhibitor和si-NC处理）和穿心莲内酯+miR-218-5p inhibitor+si-SOX5组（15 μmol·L^-1穿心莲内酯+共转染miR-218-5p inhibitor和si-SOX5处理）。用定量逆转录聚合酶链反应（qRT-PCR）检测miR-218-5p与SOX5 mRNA的相对表达水平；用5-乙炔基-2′-脱氧尿苷（EdU）法检测细胞增殖能力，用Transwell法检测细胞侵袭能力，用蛋白质印迹法检测EMT相关蛋白的相对表达水平。

结果

经过不同时间处理后，空白组、穿心莲内酯组、穿心莲内酯+inhibitor NC组和穿心莲内酯+miR-218-5p inhibitor组中miR-218-5p相对表达水平分别为1.00±0.11、2.76±0.48、2.69±0.50和1.19±0.17，SOX5 mRNA的相对表达水平分别为1.00±0.09、0.36±0.07、0.35±0.05和0.93±0.18。与空白组相比，穿心莲内酯组的miR-218-5p相对表达水平显著上调，SOX5 mRNA相对表达水平显著下调（均P<0.05）。空白组、穿心莲内酯组、穿心莲内酯+inhibitor NC组、穿心莲内酯+miR-218-5p inhibitor组、穿心莲内酯+miR-218-5p inhibitor+si-NC组和穿心莲内酯+miR-218-5p inhibitor+si-SOX5组的增殖率分别为（62.76±8.14）%、（23.58±2.87）%、（25.12±3.64）%、（51.29±9.12）%、（50.76±8.29）%和（34.18±6.83）%，侵袭个数分别为（154.23±17.24）、（67.13±9.37）、（72.81±11.28）、（125.36±18.74）、（118.15±19.80）和（80.44±12.13）个，E-钙黏蛋白（E-cadherin）相对表达水平分别为1.00±0.12、2.62±0.43、2.56±0.47、1.47±0.27、1.51±0.23和2.24±0.43，神经钙黏蛋白（N-cadherin）相对表达水平分别为1.00±0.11、0.41±0.08、0.39±0.06、0.83±0.17、0.86±0.15和0.51±0.10，波形蛋白（Vimentin）相对表达水平分别为1.00±0.08、0.49±0.07、0.46±0.09、0.85±0.16、0.88±0.17和0.55±0.11。与空白组相比，穿心莲内酯组的增殖率、侵袭个数及N-cadherin与Vimentin相对表达水平均显著降低，E-cadherin相对表达水平显著升高（均P<0.05）。

结论

穿心莲内酯可抑制口腔鳞状细胞癌的增殖、侵袭和EMT，其机制可能与上调miR-218-5p从而靶向抑制SOX5的表达有关。

关键词

穿心莲内酯 / 口腔鳞状细胞癌 / 增殖 / 侵袭 / 上皮间质转化

Abstract

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Objective

To investigate the effects of andrographolide on microRNA (miR) -218-5p/RY box transcription factor 5 (SOX5) on the proliferation, invasion and epithelial mesenchymal transformation (EMT) of oral squamous cell carcinoma cells.

Methods

CAL-27 cells were divided into the following groups: blank group (conventional culture), andrographolide group (treated with 15 μmol·L^-1 andrographolide), andrographolide + inhibitor NC group (treated with 15 μmol·L^-1 andrographolide and transfected with inhibitor NC), andrographolide + miR-218-5p inhibitor group (treated with 15 μmol·L^-1 andrographolide and transfected with miR-218-5p inhibitor), andrographolide + miR-218-5p inhibitor + si-NC group (treated with 15 μmol·L^-1 andrographolide and co-transfected with miR-218-5p inhibitor and si-NC), and andrographolide + miR-218-5p inhibitor + si-SOX5 group (treated with 15 μmol·L^-1 andrographolide and co-transfected with miR-218-5p inhibitor and si-SOX5). The relative expression levels of miR-218-5p and SOX5 mRNA were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR), cell proliferation ability was measured using the 5-ethynyl-2′-deoxyuridine (EdU) assay, cell invasion ability was assessed by Transwell assay, and the relative expression levels of EMT-related proteins were determined by Western blotting.

Results

After different treatment periods, the relative expression levels of miR-218-5p in blank group, andrographolide group, andrographolide + inhibitor NC group and andrographolide + miR-218-5p inhibitor group were 1.00±0.11, 2.76±0.48, 2.69±0.50 and 1.19±0.17, respectively; the relative expression levels of SOX5 mRNA were 1.00±0.09, 0.36±0.07, 0.35±0.05 and 0.93±0.18, respectively. Compared with the blank group, the andrographolide group showed statistically significantly upregulated miR-218-5p relative expression levels and statistically significantly reduced SOX5 mRNA relative expression levels (all P<0.05). The proliferation rates in blank group, andrographolide group, andrographolide + inhibitor NC group, andrographolide + miR-218-5p inhibitor group, andrographolide + miR-218-5p inhibitor + si-NC group and andrographolide + miR-218-5p inhibitor + si-SOX5 group were (62.76±8.14)%, (23.58±2.87)%, (25.12±3.64)%, (51.29±9.12)%, (50.76±8.29)% and (34.18±6.83)%, respectively; the number of invasive cells were (154.23±17.24), (67.13±9.37), (72.81±11.28), (125.36±18.74), (118.15±19.80) and (80.44±12.13), respectively; the relative expression levels of E-cadherin were 1.00±0.12, 2.62±0.43, 2.56±0.47, 1.47±0.27, 1.51±0.23 and 2.24±0.43, respectively; the relative expression levels of N-cadherin were 1.00±0.11, 0.41±0.08, 0.39±0.06, 0.83±0.17, 0.86±0.15 and 0.51±0.10, respectively; the relative expression levels of Vimentin were 1.00±0.08, 0.49±0.07, 0.46±0.09, 0.85±0.16, 0.88±0.17 and 0.55±0.11, respectively. Compared with blank group, andrographolide group showed statistically significantly decreased proliferation rate, number of invasive cells and relative expression levels of N-cadherin and Vimentin, while demonstrating statistically significantly increased relative expression levels of E-cadherin (all P<0.05).

Conclusion

Andrographolide inhibits the proliferation, invasion, and EMT in oral squamous cell carcinoma, potentially through upregulating miR-218-5p and subsequently targeting the suppression of SOX5 expression.

Key words

andrographolide / oral squamous cell carcinoma / proliferation / invade and attack / epithelial mesenchymal transformation

引用本文

张芳英, 黄新帮, 黄迪, 曾以旺, 黄根生. 穿心莲内酯调节miR-218-5p/SOX5影响口腔鳞状细胞癌细胞上皮间质转化的研究. 中国临床药理学杂志, 2025 , 41 (19) : 2767 -2773 . DOI: 10.13699/j.cnki.1001-6821.2025.19.012

Fang-ying ZHANG, Xin-bang HUANG, Di HUANG, Yi-wang ZENG, Gen-sheng HUANG. Research of andrographolide modulates epithelial-mesenchymal transition in oral squamous cell carcinoma by miR-218-5p/SOX5 pathways[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (19) : 2767 -2773 . DOI: 10.13699/j.cnki.1001-6821.2025.19.012

基金

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江西省卫生健康委科技计划基金资助项目(SKJP220236690)

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2025年第41卷第19期

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文章信息

doi: 10.13699/j.cnki.1001-6821.2025.19.012

接收时间：2025-05-06
首发时间：2026-08-05
出版时间：2025-10-17

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收稿日期：2025-05-06

基金

江西省卫生健康委科技计划基金资助项目(SKJP220236690)

作者信息

^a.赣州市人民医院，药剂科，江西赣州 341000

^b.赣州市人民医院，口腔颌面外科，江西赣州 341000

^c.赣州市人民医院，口腔科，江西赣州 341000

通讯作者:

黄根生，副主任医师 MP: 15879748676 E-mail: zfy50062@163.com

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https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2025.19.012

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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