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Article(id=1291404926261912257, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291404807152067353, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2025.08.019, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1730822400000, receivedDateStr=2024-11-06, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785824527563, onlineDateStr=2026-08-04, pubDate=1745769600000, pubDateStr=2025-04-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785824527563, onlineIssueDateStr=2026-08-04, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785824527563, creator=13701087609, updateTime=1785824527563, updator=13701087609, issue=Issue{id=1291404807152067353, tenantId=1146029695717560320, journalId=1246415772164075586, year='2025', volume='41', issue='8', pageStart='1051', pageEnd='1200', issueExtLink='null', onlineDate='null', pubDate='1745769600000', pubDateStr='2025-04-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785824499166, creator='13701087609', updateTime=1785824499166, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=1157, endPage=1163, ext={EN=ArticleExt(id=1291404926496793282, articleId=1291404926261912257, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Analysis of tissue distribution of 16 functional components of Gardenia jasminoides Ellis based on LC-MS/MS in mice, columnId=1246531413395263850, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Research Method, runingTitle=null, highlight=null, articleAbstract=
Objective

To develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying 16 bioactive constituents of Gardenia jasminoides Eills in various murine tissues.

Methods

The ileum, colon, stomach, liver, kidney and brain tissues of mice were collected following intragastric administration of Gardenia jasminoides Ellis. Subsequently, the samples were processed by precipitating protein. The analysis was performed using the Waters CORTECS C18 column (4.6 mm×150.0 mm, 2.7 μm) with mobile phase gradient elution consisting of a methanol-water solution, and specnuezhenide was used as the internal standard; the flow rate was set at 0.4 mL·min-1 while maintaining a column temperature of 40 ℃; detection was performed in negative ion mode utilizing an electrospray ion source. Furthermore, the method was evaluated for its specificity, standard curve and lower limit of quantification, precision and recovery, stability and reproducibility. Additionally, the tissue distribution characteristics of the active components of Gardenia jasminoides Ellis in mice were investigated.

Results

This study established a method for the analysis of 16 bioactive constituents in Gardenia jasminoides Ellis within complex matrices. The linear range spanned from 0.98 to 6.00×104 ng·mL-1, with a limit of quantitation ranging from 0.92 to 11.72 ng·mL-1, and the RSD values for intra-day and inter-day precision as well as recovery were all below 15%. Amongst the examined tissues, ileum tissue exhibited the highest levels of these active components, followed by gastric tissue. Iridoid components demonstrated extensive distribution across various tissues. The diterpenoid constituent crocetin was predominantly found in the liver and brain, while crocin Ⅱ showed higher distribution in the colon.

Conclusion

The method exhibits high specificity, excellent sensitivity, reproducibility, and stability. Moreover, the matrix effect can be controlled effectively, rendering it suitable for analyzing Gardenia jasminoides Ellis in complex matrices. The intestine is the major tissue for the distribution of Gardenia jasminoides Ellis, which may be an important part for its efficacy. Furthermore, the extensive pharmacological effects of Gardenia jasminoides Ellis may be related to the component tendency to different tissues.

, authors=Jia-qi QIN1, Zhi RAO2, Dong-xia LÜ2, Fan ZHANG2, Xin-yi LUO2, Yu-hui WEI1, 2, authorsList=Jia-qi QIN, Zhi RAO, Dong-xia LÜ, Fan ZHANG, Xin-yi LUO, Yu-hui WEI, authorCompany=null, correspAuthors=Yu-hui WEI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1291404926912029379, articleId=1291404926261912257, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=基于LC-MS/MS法分析栀子16种功效成分的小鼠组织分布, columnId=1246531413600784769, journalTitle=中国临床药理学杂志, columnName=研究方法, runingTitle=null, highlight=null, articleAbstract=
目的

建立小鼠体内不同组织中栀子16种功效成分测定的液相色谱-质谱联用（LC-MS/MS）分析方法。

方法

小鼠灌胃给予栀子后取回肠、结肠、胃、肝、肾、脑组织，用沉淀蛋白法处理样品，Waters CORTECS C18色谱柱（4.6 mm×150.0 mm，2.7 μm），流动相甲醇-水溶液进行梯度洗脱，内标物为特女贞苷，流速0.4 mL·min-1，柱温40 ℃，电喷雾离子源，负离子模式检测。考察该方法的专属性、标准曲线和定量下限、精密度与回收率、稳定性以及重现性，并考察栀子功效成分在小鼠体内的组织分布特征。

结果

本研究建立了16种栀子功效成分在复杂基质中的分析方法，其线性范围为0.98~6.00×104 ng·mL-1，定量下限为0.92~11.72 ng·mL-1，日内、日间精密度及回收率RSD值均小于15%。16种功效成分在回肠组织中均有较高含量，胃组织次之。环烯醚萜类成分在各组织中均有较高分布，二萜类成分西红花酸主要分布于肝、脑中，而西红花苷Ⅱ主要分布于结肠中。

结论

本方法专属性高，灵敏度、重现性及稳定性较好，且基质效应可控，适用于进行复杂基质中栀子成分分析研究。栀子成分群主要分布于肠道，这可能是其发挥功效的重要部位，其药理作用可能与其成分在不同组织中的趋向性相关。

, authors=秦佳琪1, 饶志2, 吕东霞2, 张帆2, 雒馨怡2, 魏玉辉1, 2, authorsList=秦佳琪, 饶志, 吕东霞, 张帆, 雒馨怡, 魏玉辉, authorCompany=null, correspAuthors=魏玉辉, authorNote=

秦佳琪（1998-），女，硕士研究生，主要从事中药药理及机制研究

, correspAuthorsNote=
魏玉辉，教授，博士生导师 Tel: （0931）8356854 E-mail:
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基于LC-MS/MS法分析栀子16种功效成分的小鼠组织分布
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秦佳琪 1 , 饶志 2 , 吕东霞 2 , 张帆 2 , 雒馨怡 2 , 魏玉辉 1, 2
中国临床药理学杂志 | 研究方法 2025,41(8): 1157-1163
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中国临床药理学杂志 |研究方法 2025 , 41 (8) : 1157 -1163
基于LC-MS/MS法分析栀子16种功效成分的小鼠组织分布
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秦佳琪1, 饶志2, 吕东霞2, 张帆2, 雒馨怡2, 魏玉辉1, 2
作者信息
  • 1.兰州大学 药学院，甘肃 兰州 730000
  • 2.兰州大学 第一医院 药剂科，甘肃 兰州 730000
通讯作者:
魏玉辉，教授，博士生导师 Tel: （0931）8356854 E-mail:
作者简介:

秦佳琪（1998-），女，硕士研究生，主要从事中药药理及机制研究

Analysis of tissue distribution of 16 functional components of Gardenia jasminoides Ellis based on LC-MS/MS in mice
Jia-qi QIN1, Zhi RAO2, Dong-xia LÜ2, Fan ZHANG2, Xin-yi LUO2, Yu-hui WEI1, 2
Affiliations
  • 1.College of Pharmacy, Lanzhou University, Lanzhou 730000, Gansu Province, China
  • 2.Department of Pharmacy, First Hosiptal of Lanzhou University, Lanzhou 730000, Gansu Province, China
出版时间: 2025-04-28 doi: 10.13699/j.cnki.1001-6821.2025.08.019
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摘要
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目的

建立小鼠体内不同组织中栀子16种功效成分测定的液相色谱-质谱联用（LC-MS/MS）分析方法。

方法

小鼠灌胃给予栀子后取回肠、结肠、胃、肝、肾、脑组织，用沉淀蛋白法处理样品，Waters CORTECS C18色谱柱（4.6 mm×150.0 mm，2.7 μm），流动相甲醇-水溶液进行梯度洗脱，内标物为特女贞苷，流速0.4 mL·min-1，柱温40 ℃，电喷雾离子源，负离子模式检测。考察该方法的专属性、标准曲线和定量下限、精密度与回收率、稳定性以及重现性，并考察栀子功效成分在小鼠体内的组织分布特征。

结果

本研究建立了16种栀子功效成分在复杂基质中的分析方法，其线性范围为0.98~6.00×104 ng·mL-1，定量下限为0.92~11.72 ng·mL-1，日内、日间精密度及回收率RSD值均小于15%。16种功效成分在回肠组织中均有较高含量，胃组织次之。环烯醚萜类成分在各组织中均有较高分布，二萜类成分西红花酸主要分布于肝、脑中，而西红花苷Ⅱ主要分布于结肠中。

结论

本方法专属性高，灵敏度、重现性及稳定性较好，且基质效应可控，适用于进行复杂基质中栀子成分分析研究。栀子成分群主要分布于肠道，这可能是其发挥功效的重要部位，其药理作用可能与其成分在不同组织中的趋向性相关。

栀子  /  环烯醚萜类  /  二萜类  /  液相色谱-质谱联用仪  /  功效成分  /  组织分布
Objective

To develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying 16 bioactive constituents of Gardenia jasminoides Eills in various murine tissues.

Methods

The ileum, colon, stomach, liver, kidney and brain tissues of mice were collected following intragastric administration of Gardenia jasminoides Ellis. Subsequently, the samples were processed by precipitating protein. The analysis was performed using the Waters CORTECS C18 column (4.6 mm×150.0 mm, 2.7 μm) with mobile phase gradient elution consisting of a methanol-water solution, and specnuezhenide was used as the internal standard; the flow rate was set at 0.4 mL·min-1 while maintaining a column temperature of 40 ℃; detection was performed in negative ion mode utilizing an electrospray ion source. Furthermore, the method was evaluated for its specificity, standard curve and lower limit of quantification, precision and recovery, stability and reproducibility. Additionally, the tissue distribution characteristics of the active components of Gardenia jasminoides Ellis in mice were investigated.

Results

This study established a method for the analysis of 16 bioactive constituents in Gardenia jasminoides Ellis within complex matrices. The linear range spanned from 0.98 to 6.00×104 ng·mL-1, with a limit of quantitation ranging from 0.92 to 11.72 ng·mL-1, and the RSD values for intra-day and inter-day precision as well as recovery were all below 15%. Amongst the examined tissues, ileum tissue exhibited the highest levels of these active components, followed by gastric tissue. Iridoid components demonstrated extensive distribution across various tissues. The diterpenoid constituent crocetin was predominantly found in the liver and brain, while crocin Ⅱ showed higher distribution in the colon.

Conclusion

The method exhibits high specificity, excellent sensitivity, reproducibility, and stability. Moreover, the matrix effect can be controlled effectively, rendering it suitable for analyzing Gardenia jasminoides Ellis in complex matrices. The intestine is the major tissue for the distribution of Gardenia jasminoides Ellis, which may be an important part for its efficacy. Furthermore, the extensive pharmacological effects of Gardenia jasminoides Ellis may be related to the component tendency to different tissues.

Gardenia jasminoides Ellis  /  iridoids  /  diterpenoids  /  liquid chromatography-tandem mass spectrometry  /  active component  /  tissue distributio
秦佳琪, 饶志, 吕东霞, 张帆, 雒馨怡, 魏玉辉. 基于LC-MS/MS法分析栀子16种功效成分的小鼠组织分布. 中国临床药理学杂志, 2025 , 41 (8) : 1157 -1163 . DOI: 10.13699/j.cnki.1001-6821.2025.08.019
Jia-qi QIN, Zhi RAO, Dong-xia LÜ, Fan ZHANG, Xin-yi LUO, Yu-hui WEI. Analysis of tissue distribution of 16 functional components of Gardenia jasminoides Ellis based on LC-MS/MS in mice[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (8) : 1157 -1163 . DOI: 10.13699/j.cnki.1001-6821.2025.08.019
基金
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  • 国家自然科学基金资助项目(82174067; 82460856)
  • 甘肃省卫生健康委员会中医药科研课题资助项目(GZKP-2O22-33)
  • 甘肃省联合科研基金资助项目(23JRRA1492)
  • 兰州大学第一医院院内基金资助项目(ldyyyn2023-34)
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2025年第41卷第8期
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文章信息
doi: 10.13699/j.cnki.1001-6821.2025.08.019
  • 接收时间：2024-11-06
  • 首发时间：2026-08-04
  • 出版时间：2025-04-28
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出版历史
  • 收稿日期：2024-11-06
基金
国家自然科学基金资助项目(82174067; 82460856)
甘肃省卫生健康委员会中医药科研课题资助项目(GZKP-2O22-33)
甘肃省联合科研基金资助项目(23JRRA1492)
兰州大学第一医院院内基金资助项目(ldyyyn2023-34)
作者信息
    1.兰州大学 药学院，甘肃 兰州 730000
    2.兰州大学 第一医院 药剂科，甘肃 兰州 730000

通讯作者:

魏玉辉，教授，博士生导师 Tel: （0931）8356854 E-mail:
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https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2025.08.019
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2种不同金属材料的力学参数
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Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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