Article(id=1291404706618798697, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291404681641713991, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2025.07.013, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1729008000000, receivedDateStr=2024-10-16, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785824475197, onlineDateStr=2026-08-04, pubDate=1744819200000, pubDateStr=2025-04-17, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785824475197, onlineIssueDateStr=2026-08-04, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785824475197, creator=13701087609, updateTime=1785824475197, updator=13701087609, issue=Issue{id=1291404681641713991, tenantId=1146029695717560320, journalId=1246415772164075586, year='2025', volume='41', issue='7', pageStart='901', pageEnd='1050', issueExtLink='null', onlineDate='null', pubDate='1744819200000', pubDateStr='2025-04-17', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785824469242, creator='13701087609', updateTime=1785897128388, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1291709436255555700, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291404681641713991, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1291709436255555701, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291404681641713991, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=967, endPage=971, ext={EN=ArticleExt(id=1291404706794959466, articleId=1291404706618798697, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Effects of Banxia-Huanglian ameliorating diabetic gastroparesis rats based on the Akt/GSK-3 signaling pathway, columnId=1246531407326105792, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Clinical and Basic Bridging Research, runingTitle=null, highlight=null, articleAbstract=
Objective

To investigate the mechanism of Banxia-Huanglian (BX-HL) in improving diabetic gastroparesis based on dopaminergic synaptic pathway.

Methods

The possible signaling pathways of BX-HL on the treatment of diabetic gastroparesis (DGP) were carried out by network pharmacology methods. The DGP model was constructed by intraperitoneal injection of 60 mg·kg-1 streptozotocin (STZ) + high-sugar and high-fat diet. The DGP rats were randomly divided into model group and experimental group, with 10 rats in each group; another 10 normal rats were taken as the normal group. The experimental group was administered by gavage with BX-HL solution at a dose of 1.35 g·kg·d-1, while the normal group and model group were administered by gavage with an equal volume of 0.9% NaCl once a day. The rats in the three groups were administered once a day for 21 days. The gastric emptying rate and intestinal propulsion rate of rats were measured by activated charcoal administration, the levels of neurotransmitter dopamine (DA) in brain tissues were determined by enzyme-linked immunosorbent assay, and the expression levels of protein kinase B (Akt) and glycogen synthase kinase-3 (GSK-3) proteins in brain tissues were determined by Western blotting.

Results

The effective components and corresponding signaling pathways, such as dopaminergic synapses, were screened by various databases for BX-HL on the treatment of diabetic gastroparesis. The fasting blood glucose levels in the normal group, model group and experimental group were (5.61±0.36), (23.40±1.58) and (6.18±0.42) mmol·L-1; the gastric emptying rates were (71.33±1.23)%, (35.43±3.12)% and (61.59±4.66)%; the intestinal transit rates were (53.19±3.48)%, (29.33±1.91)% and (38.53±2.80)%; the DA contents in brain tissue were (42.43±2.97), (88.20±8.46) and (52.64±5.03) pmol·L-1; the relative protein expression levels of Akt were 0.67±0.00, 1.35±0.04 and 0.94±0.01; the relative protein expression levels of GSK-3 were 0.96±0.01, 1.24±0.05 and 0.91±0.01, respectively. The above indexes of the experimental group showed statistical significance compared to the model group (all P<0.001).

Conclusion

BX-HL has an ameliorative effect on diabetic gastroparesis in rats, which is mainly reflected in the lowering of blood glucose, the promotion of gastric emptying and intestinal propulsion, the reduction of neurotransmitter DA level, its mechanism may be related to inhibite the Akt/GSK-3 protein expression of dopaminergic synaptic pathway.

, authors=Qiong CHEN1, 2, Jia-yi HUANG1, 2, Xian-min SHEN1, 2, Lu-rong ZHANG1, 2, Fei WANG1, 2, Heng XU1, 2, authorsList=Qiong CHEN, Jia-yi HUANG, Xian-min SHEN, Lu-rong ZHANG, Fei WANG, Heng XU, authorCompany=null, correspAuthors=Fei WANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1291404707075977835, articleId=1291404706618798697, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=基于Akt/GSK-3信号通路探讨半夏-黄连改善糖尿病胃轻瘫大鼠的作用, columnId=1246531407485489349, journalTitle=中国临床药理学杂志, columnName=临床与基础桥接研究, runingTitle=null, highlight=null, articleAbstract=
目的

基于多巴胺能突触通路探讨半夏-黄连改善糖尿病胃轻瘫(DGP)的作用机制。

方法

用网络药理学方法对半夏-黄连治疗糖尿病胃轻瘫的信号通路进行分析。用腹腔注射60 mg·kg-1链脲佐菌素+高糖高脂饲料饲养构建DGP模型。将造模成功的DGP大鼠随机分为模型组和实验组,每组10只;另取10只正常大鼠作为正常组。实验组灌胃给予1.35 g·kg-1·d-1的半夏-黄连溶液,正常组和模型组均灌胃给予等体积0.9% NaCl。3组大鼠每天给药1次,连续给药21 d。用灌服活性炭的方法测量大鼠的胃排空率和肠推进率,用酶联免疫吸附试验法测定脑组织中神经递质多巴胺(DA)的水平,用蛋白质印迹法测定脑组织中蛋白激酶B(Akt)和糖原合成酶激酶-3(GSK-3)蛋白的表达水平。

结果

通过多种数据库共筛选得到半夏-黄连治疗糖尿病胃轻瘫主要通过多巴胺能突触等多条信号通路。正常组、模型组和实验组的空腹血糖水平分别为(5.61±0.36)、(23.40±1.58)和(6.18±0.42)mmol·L-1,胃排空率分别为(71.33±1.23)%、(35.43±3.12)%和(61.59±4.66)%,肠推进率分别为(53.19±3.48)%、(29.33±1.91)%和(38.53±2.80)%,脑组织匀浆液中DA含量分别为(42.43±2.97)、(88.20±8.46)和(52.64±5.03)pmol·L-1,Akt蛋白相对表达水平分别为0.67±0.00、1.35±0.04和0.94±0.01,GSK-3蛋白相对表达水平分别为0.96±0.01、1.24±0.05和0.91±0.01。实验组的上述指标与模型组相比,在统计学上差异均有统计学意义(均P<0.001)。

结论

半夏-黄连对糖尿病胃轻瘫大鼠具有改善作用,其主要体现在降低空腹血糖、促进胃排空和肠推进,降低脑组织DA水平,其作用机制可能是通过抑制多巴胺能突触通路Akt/GSK-3蛋白表达来发挥作用。

, authors=陈琼1, 2, 黄佳怡1, 2, 沈贤敏1, 2, 张露蓉1, 2, 王斐1, 2, 许恒1, 2, authorsList=陈琼, 黄佳怡, 沈贤敏, 张露蓉, 王斐, 许恒, authorCompany=null, correspAuthors=王斐, authorNote=

陈琼(2001-),女,硕士研究生,主要从事中药防治糖尿病及其并发症的机制研究

, correspAuthorsNote=
王斐,研究员,硕士生导师 Tel: (0512)67872504 E-mail:
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基于Akt/GSK-3信号通路探讨半夏-黄连改善糖尿病胃轻瘫大鼠的作用
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陈琼 1, 2 , 黄佳怡 1, 2 , 沈贤敏 1, 2 , 张露蓉 1, 2 , 王斐 1, 2 , 许恒 1, 2
中国临床药理学杂志 | 临床与基础桥接研究 2025,41(7): 967-971
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中国临床药理学杂志 |临床与基础桥接研究 2025 , 41 (7) : 967 -971
基于Akt/GSK-3信号通路探讨半夏-黄连改善糖尿病胃轻瘫大鼠的作用
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陈琼1, 2, 黄佳怡1, 2, 沈贤敏1, 2, 张露蓉1, 2, 王斐1, 2 , 许恒1, 2
作者信息
  • 1.南京中医药大学 附属苏州市中医医院 中心实验室,江苏 苏州 215009
  • 2.苏州市吴门医派研究院 中药临床药学实验室,江苏 苏州 215009
通讯作者:
王斐,研究员,硕士生导师 Tel: (0512)67872504 E-mail:
作者简介:

陈琼(2001-),女,硕士研究生,主要从事中药防治糖尿病及其并发症的机制研究

Effects of Banxia-Huanglian ameliorating diabetic gastroparesis rats based on the Akt/GSK-3 signaling pathway
Qiong CHEN1, 2, Jia-yi HUANG1, 2, Xian-min SHEN1, 2, Lu-rong ZHANG1, 2, Fei WANG1, 2 , Heng XU1, 2
Affiliations
  • 1.Central Laboratory, Suzhou Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou 215009, Jiangsu Province, China
  • 2.Clinical Pharmaceutical Laboratory of Traditional Chinese Medicine, Suzhou Academy of Wumen Chinese Medicine, Suzhou 215009, Jiangsu Province, China
出版时间: 2025-04-17 doi: 10.13699/j.cnki.1001-6821.2025.07.013
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目的

基于多巴胺能突触通路探讨半夏-黄连改善糖尿病胃轻瘫(DGP)的作用机制。

方法

用网络药理学方法对半夏-黄连治疗糖尿病胃轻瘫的信号通路进行分析。用腹腔注射60 mg·kg-1链脲佐菌素+高糖高脂饲料饲养构建DGP模型。将造模成功的DGP大鼠随机分为模型组和实验组,每组10只;另取10只正常大鼠作为正常组。实验组灌胃给予1.35 g·kg-1·d-1的半夏-黄连溶液,正常组和模型组均灌胃给予等体积0.9% NaCl。3组大鼠每天给药1次,连续给药21 d。用灌服活性炭的方法测量大鼠的胃排空率和肠推进率,用酶联免疫吸附试验法测定脑组织中神经递质多巴胺(DA)的水平,用蛋白质印迹法测定脑组织中蛋白激酶B(Akt)和糖原合成酶激酶-3(GSK-3)蛋白的表达水平。

结果

通过多种数据库共筛选得到半夏-黄连治疗糖尿病胃轻瘫主要通过多巴胺能突触等多条信号通路。正常组、模型组和实验组的空腹血糖水平分别为(5.61±0.36)、(23.40±1.58)和(6.18±0.42)mmol·L-1,胃排空率分别为(71.33±1.23)%、(35.43±3.12)%和(61.59±4.66)%,肠推进率分别为(53.19±3.48)%、(29.33±1.91)%和(38.53±2.80)%,脑组织匀浆液中DA含量分别为(42.43±2.97)、(88.20±8.46)和(52.64±5.03)pmol·L-1,Akt蛋白相对表达水平分别为0.67±0.00、1.35±0.04和0.94±0.01,GSK-3蛋白相对表达水平分别为0.96±0.01、1.24±0.05和0.91±0.01。实验组的上述指标与模型组相比,在统计学上差异均有统计学意义(均P<0.001)。

结论

半夏-黄连对糖尿病胃轻瘫大鼠具有改善作用,其主要体现在降低空腹血糖、促进胃排空和肠推进,降低脑组织DA水平,其作用机制可能是通过抑制多巴胺能突触通路Akt/GSK-3蛋白表达来发挥作用。

半夏-黄连  /  多巴胺  /  脑肠轴  /  糖尿病胃轻瘫  /  糖原合成酶激酶-3  /  网络药理学
Objective

To investigate the mechanism of Banxia-Huanglian (BX-HL) in improving diabetic gastroparesis based on dopaminergic synaptic pathway.

Methods

The possible signaling pathways of BX-HL on the treatment of diabetic gastroparesis (DGP) were carried out by network pharmacology methods. The DGP model was constructed by intraperitoneal injection of 60 mg·kg-1 streptozotocin (STZ) + high-sugar and high-fat diet. The DGP rats were randomly divided into model group and experimental group, with 10 rats in each group; another 10 normal rats were taken as the normal group. The experimental group was administered by gavage with BX-HL solution at a dose of 1.35 g·kg·d-1, while the normal group and model group were administered by gavage with an equal volume of 0.9% NaCl once a day. The rats in the three groups were administered once a day for 21 days. The gastric emptying rate and intestinal propulsion rate of rats were measured by activated charcoal administration, the levels of neurotransmitter dopamine (DA) in brain tissues were determined by enzyme-linked immunosorbent assay, and the expression levels of protein kinase B (Akt) and glycogen synthase kinase-3 (GSK-3) proteins in brain tissues were determined by Western blotting.

Results

The effective components and corresponding signaling pathways, such as dopaminergic synapses, were screened by various databases for BX-HL on the treatment of diabetic gastroparesis. The fasting blood glucose levels in the normal group, model group and experimental group were (5.61±0.36), (23.40±1.58) and (6.18±0.42) mmol·L-1; the gastric emptying rates were (71.33±1.23)%, (35.43±3.12)% and (61.59±4.66)%; the intestinal transit rates were (53.19±3.48)%, (29.33±1.91)% and (38.53±2.80)%; the DA contents in brain tissue were (42.43±2.97), (88.20±8.46) and (52.64±5.03) pmol·L-1; the relative protein expression levels of Akt were 0.67±0.00, 1.35±0.04 and 0.94±0.01; the relative protein expression levels of GSK-3 were 0.96±0.01, 1.24±0.05 and 0.91±0.01, respectively. The above indexes of the experimental group showed statistical significance compared to the model group (all P<0.001).

Conclusion

BX-HL has an ameliorative effect on diabetic gastroparesis in rats, which is mainly reflected in the lowering of blood glucose, the promotion of gastric emptying and intestinal propulsion, the reduction of neurotransmitter DA level, its mechanism may be related to inhibite the Akt/GSK-3 protein expression of dopaminergic synaptic pathway.

Banxia-Huanglian  /  dopamine  /  brain-gut axis  /  diabetic gastroparesis  /  glycogen synthase kinase-3  /  network pharmacology
陈琼, 黄佳怡, 沈贤敏, 张露蓉, 王斐, 许恒. 基于Akt/GSK-3信号通路探讨半夏-黄连改善糖尿病胃轻瘫大鼠的作用. 中国临床药理学杂志, 2025 , 41 (7) : 967 -971 . DOI: 10.13699/j.cnki.1001-6821.2025.07.013
Qiong CHEN, Jia-yi HUANG, Xian-min SHEN, Lu-rong ZHANG, Fei WANG, Heng XU. Effects of Banxia-Huanglian ameliorating diabetic gastroparesis rats based on the Akt/GSK-3 signaling pathway[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (7) : 967 -971 . DOI: 10.13699/j.cnki.1001-6821.2025.07.013
  • 国家自然科学基金青年科学基金资助项目(81903899)
  • 苏州市姑苏卫生人才计划基金资助项目(GSWS2020089)
  • 苏州市科技发展计划基金资助项目(SKY2023217; SKYD2023155)
  • 中医骨伤重点实验室建设基金资助项目(JSDW202253; SZS2022019)
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doi: 10.13699/j.cnki.1001-6821.2025.07.013
  • 接收时间:2024-10-16
  • 首发时间:2026-08-04
  • 出版时间:2025-04-17
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  • 收稿日期:2024-10-16
基金
国家自然科学基金青年科学基金资助项目(81903899)
苏州市姑苏卫生人才计划基金资助项目(GSWS2020089)
苏州市科技发展计划基金资助项目(SKY2023217; SKYD2023155)
中医骨伤重点实验室建设基金资助项目(JSDW202253; SZS2022019)
作者信息
    1.南京中医药大学 附属苏州市中医医院 中心实验室,江苏 苏州 215009
    2.苏州市吴门医派研究院 中药临床药学实验室,江苏 苏州 215009

通讯作者:

王斐,研究员,硕士生导师 Tel: (0512)67872504 E-mail:
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2种不同金属材料的力学参数

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Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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