中国临床药理学杂志

circRNA_0044556在紫杉醇耐药三阴性乳腺癌中的作用研究

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陈晶晶 ^1a , 石鹏 ^1b , 王宏 ^1a , 常猛 ^1a , 蒋楠 ^1a , 马杰 ^1a

中国临床药理学杂志 | 临床与基础桥接研究 2025,41(3): 345-349

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中国临床药理学杂志 |临床与基础桥接研究 2025 , 41 (3) : 345 -349

circRNA_0044556在紫杉醇耐药三阴性乳腺癌中的作用研究

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陈晶晶^1a, 石鹏^1b, 王宏^1a, 常猛^1a, 蒋楠^1a, 马杰^1a

作者信息

^1a.唐山市人民医院，乳腺一科，河北唐山 063000

^1b.唐山市人民医院，泌尿外二科，河北唐山 063000

通讯作者:

陈晶晶，副主任医师 MP: 13323150163 E-mail: crystalchen201504@163.com

作者简介:

陈晶晶（1984-），女，副主任医师，主要从事乳腺肿瘤学的研究

Effects of circRNA_0044556 on paclitaxel resistance in triple-negative breast cancer

Jing-jing CHEN^1a, Peng SHI^1b, Hong WANG^1a, Meng CHANG^1a, Nan JIANG^1a, Jie MA^1a

Affiliations

^1a.First Department of Breast, Tangshan People’s Hospital, Tangshan 063000, Hebei Province, China

^1b.Second Department of Urology, Tangshan People’s Hospital, Tangshan 063000, Hebei Province, China

出版时间: 2025-02-17 doi: 10.13699/j.cnki.1001-6821.2025.03.010

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摘要

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目的

探究circRNA_0044556调节三阴性乳腺癌（TNBC）对紫杉醇（PTX）的抵抗以及耐药性的机制。

方法

将人TNBC细胞系（MDA-MB-231）通过暴露于浓度持续增加的PTX中，获得PTX抗性TNBC细胞（MDA-MB-231/PTX）。选对数生长期的MDA-MB-231和MDA-MB-231/PTX细胞进行分组实验，分别转染靶向circRNA_0044556的短发夹RNA（sh-circRNA_0044556）及阴性对照（sh-NC），分为MDA-MB-231-NC组、MDA-MB-231-circRNA_0044556组、MDA-MB-231/PTX-NC组和MDA-MB-231/PTX-circRNA_0044556组。用实时荧光定量聚合酶链反应（RT-qPCR）测定细胞中circRNA_0044556的表达水平，用四甲基偶氮唑蓝（MTT）和克隆形成实验检测细胞增殖能力，用流式细胞术检测细胞凋亡能力，用蛋白质印迹法检测细胞增殖、凋亡相关蛋白质的表达情况。

结果

circRNA_0044556在MDA-MB-231/PTX、MDA-MB-231及MCF-10A细胞中的相对表达水平分别为6.08±0.59、3.36±0.11和1.00±0.02，细胞间比较，在统计学上差异均有统计学意义（均P<0.001）。MDA-MB-231-NC组和MDA-MB-231-circRNA_0044556组增殖率分别为（100.00±0.84）%和（54.38±1.20）%，细胞凋亡率分别为（2.41±0.34）%和（21.65±0.29）%，在统计学上差异均有统计学意义（均P<0.001）。MDA-MB-231/PTX-NC组和MDA-MB-231/PTX-circRNA_0044556组增殖率分别为（112.29±1.19）%和（61.33±1.24）%，细胞凋亡率分别为（2.08±0.07）%和（14.38±0.16）%，MDA-MB-231/PTX-NC组和MDA-MB-231/PTX-circRNA_0044556组增殖率及凋亡率比较，在统计学上差异均有统计学意义（均P<0.001）。MDA-MB-231-NC组和MDA-MB-231-circRNA_0044556组Ki-67蛋白相对表达水平分别为1.00±0.02和0.67±0.02，B淋巴细胞瘤-2（Bcl-2）蛋白相对表达水平分别为1.00±0.02和0.64±0.01，在统计学上差异均有统计学意义（均P<0.05）。MDA-MB-231/PTX-NC组和MDA-MB-231/PTX-circRNA_0044556组Ki-67蛋白相对表达水平分别为1.00±0.03和0.81±0.01，Bax蛋白相对表达水平分别为1.00±0.02和1.55±0.02，Bcl-2蛋白相对表达水平分别为1.00±0.03和0.79±0.01，MDA-MB-231/PTX-NC组的上述指标与MDA-MB-231/PTX-circRNA_0044556比较，在统计学上差异均有统计学意义（均P<0.05）。

结论

敲低circRNA_0044556表达能够抑制TNBC细胞的恶性进展和PTX耐药性。

关键词

三阴性乳腺癌 / 环状RNA_0044556 / 增殖 / 凋亡 / 紫杉醇抵抗

Abstract

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Objective

To investigate the effect of circRNA_0044556 on the resistance of triple-negative breast cancer (TNBC) to paclitaxel (PTX) and the mechanism of drug resistance.

Methods

PTX resistant TNBC cells (MDA-MB-231/PTX) were obtained by exposing the human TNBC cell (MDA-MB-231) to increasing concentrations of PTX. MDA-MB-231 and MDA-MB-231/PTX cells in logarithmic growth phase were treated with circRNA_0044556 short hairpin RNA (sh-circRNA_0044556) and negative control (sh-NC), respectively. The groups were finally divided into MDA-MB-231-NC, MDA-MB-231-circRNA_0044556, MDA-MB-231/PTX-NC and MDA-MB-231/PTX-circRNA_0044556 groups. The expression level of circRNA_0044556 in cells was determined by real-time quantitative polymerase chain reaction (RT-qPCR); methyl thiazolyl tetrazolium assay and colony formation assay were used to observe cell proliferation; flow cytometry was used to observe cell apoptosisand; Western blot was used to observe the expression of cell proliferation and apoptosis-related proteins.

Results

The relative expression levels of circRNA_0044556 in MDA-MB-231/PTX, MDA-MB-231 and MCF-10A cells were 6.08±0.59, 3.36±0.11 and 1.00±0.02, respectively, and the differences were statistically significant (all P<0.001). Proliferation rates of MDA-MB-231-NC and MDA-MB-231-circRNA_0044556 groups were (100.00±0.84)% and (54.38±1.20)%; the apoptosis rates were (2.41±0.34)% and (21.65±0.29)%, respectively, and the differences were statistically significant (all P<0.001). The proliferation rates of MDA-MB-231/PTX-NC and MDA-MB-231/PTX-circRNA_0044556 groups were (112.29±1.19)% and (61.33±1.24)%; the apoptosis rates were (2.08±0.07)% and (14.38±0.16)%, respectively, the differences were all statistically significant (all P<0.001). The relative protein expression level of Ki-67 in MDA-MB-231-NC and MDA-MB-231-circRNA_0044556 groups were 1.00±0.02 and 0.67±0.02; the relative protein expression of Bcl-2-associated X protein (Bax) were 1.00±0.02 and 1.46±0.03; and the differences were statistically significant (all P<0.05). The relative relative protein expression levels of Ki-67 in MDA-MB-231/PTX-NC group and MDA-MB-231/PTX-circRNA_0044556 group were 1.00±0.03 and 0.81±0.01, respectively; the relative expression levels of Bax protein were 1.00±0.02 and 1.55±0.02, respectively; the relative expression levels of Bcl-2 protein were 1.00±0.03 and 0.79±0.01, respectively. The above indexes in MDA-MB-231/PTX-NC group were significantly different from those in MDA-MB-231/PTX-circRNA_0044556 group (all P<0.05).

Conclusions

Knocking down the expression of circRNA_0044556 can inhibit the malignant progression and PTX resistance of TNBC cells.

Key words

triple-negative breast cancer / circRNA_0044556 / proliferation / apoptosis / resistance to paclitaxel

引用本文

陈晶晶, 石鹏, 王宏, 常猛, 蒋楠, 马杰. circRNA_0044556在紫杉醇耐药三阴性乳腺癌中的作用研究. 中国临床药理学杂志, 2025 , 41 (3) : 345 -349 . DOI: 10.13699/j.cnki.1001-6821.2025.03.010

Jing-jing CHEN, Peng SHI, Hong WANG, Meng CHANG, Nan JIANG, Jie MA. Effects of circRNA_0044556 on paclitaxel resistance in triple-negative breast cancer[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (3) : 345 -349 . DOI: 10.13699/j.cnki.1001-6821.2025.03.010

基金

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河北省医学科学研究课题计划基金资助项目(20211662)

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2025年第41卷第3期

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doi: 10.13699/j.cnki.1001-6821.2025.03.010

接收时间：2024-06-25
首发时间：2026-08-04
出版时间：2025-02-17

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收稿日期：2024-06-25

基金

河北省医学科学研究课题计划基金资助项目(20211662)

作者信息

^1a.唐山市人民医院，乳腺一科，河北唐山 063000

^1b.唐山市人民医院，泌尿外二科，河北唐山 063000

通讯作者:

陈晶晶，副主任医师 MP: 13323150163 E-mail: crystalchen201504@163.com

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https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2025.03.010

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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