To prevent accidents caused by miners' unsafe behaviors induced by physical fatigue and reduce the impact of physical fatigue on safe behaviors abality, a physical fatigue response test was carried out. First, forty adult male participants were equally divided into two groups, with each group induced to develop MEF and MPF respectively. Subsequently, a series of operational performance tests were conducted to measure attention level, reaction speed, judgment accuracy, and body coordination ability. Then, paired sample t-tests were employed to analyze pre- and post-fatigue behavioral data, investigating the effects of physiological fatigue on safety-related performance capabilities. Finally, evidence-based management recommendations were formulated according to the analytical results. The findings reveal that muscular endurance fatigue significantly compromise attention levels through its effects on mean pupil diameter(P<0.01), blink frequency(P<0.01), and saccadic rate(P<0.05). While muscular explosive power fatigue also demonstrates measurable influence on attentional performance as evidenced by altered pupil diameter(P<0.01), its effect magnitude is statistically inferior to that of endurance fatigue. Also, muscular explosive power fatigue reduces the body coordination ability, as both hand-eye coordination ability(P<0.05) and fine operation ability(P<0.05)have significantly declined. However, muscle explosive fatigue does have a certain improvement effect on reaction speed, with simple reaction time increasing by 0.028 s(P<0.01) and comprehensive reaction time increasing by 0.020 s(P<0.01). Physiological fatigue has no significant impact on judgment accuracy.