Objective To investigate the chemical constituents from the methanol extract of the polyporaceae fungus Ganoderma cochlear and preliminarily evaluate their anti-atherosclerotic activity in vitro. Methods The extract was systematically isolated and purified by D-101 macroporous adsorption resin, silica gel column chromatography, reversed-phase C₁₈ column chromatography, preparative thin layer chromatography and semi-preparative HPLC. The structures of the isolated compounds were elucidated by HR-ESI-MS, IR, and NMR. Meanwhile, the absolute configurations of the new compounds were determined by NMR and ECD calculation. The in vitro anti-atherosclerotic activities of the isolates were assessed using an oxidized low-density lipoprotein (ox-LDL)-induced macrophage lipid deposition model. Results Two novel compounds were isolated and identified from G. cochlear, designated as 7,10-epoxy-3,4-seco-9 (10→19) abeo-25,26,27-trinorlanosta-4,11-dioxo-8-en-3,24-dimethyl ester (1) and 6-hydroxy-2-(4-methylpent-3-en-1-yl)-4H-chromen-4-one (2). In the ox-LDL-induced macrophage model, compounds 1 and 2 did not exhibit significant anti-lipid deposition activity at 40 μmol/L. Conclusion This study successfully isolated and identified two new compounds, and named as ganodeconoid J (1) and ganodeconoid K (2). Compound 1 represents the first Ganoderma triterpenoid possessing a 5β-H, enriching the chemical diversity of Ganoderma fungi. Compounds 1 and 2 did not show significant in vitro anti-atherosclerotic activity.

Ganoderma cochlear (Blume & T. Nees) Bres.  /  triterpenoids  /  meroterpenoids  /  ganodeconoid J  /  ganodeconoid K  /  Ganoderma triterpenoid  /  lipid deposition