Objective Study on the chemical components of Chishui Dendrobium nobile (Orchidaceae).Methods A systematic chemical study was conducted on the n-butanol fraction of the 95% ethanol extract of Chishui D. nobile using methods and techniques such as silica gel, Toyopearl HW-40C, and semi-preparative liquid chromatography, with structural identification carried out through HRESIMS, NMR, IR, UV, and other spectroscopic methods. Results A total of six compounds were isolated and identified as 6,7-dimethyl-1-[(2S,3R,4R)-2,3,5-trihydroxy-4-O-β-D-glucopyranosyl]-1,4-dihydroquinoxaline-2,3-dione (1), 2-phenylethyl 3-O-β-D-glucopyranosyl-β-D-glucopyranoside (2), diospyrososide (3), benzyl-β-D-glucoside (4), icariside D₂ (5), 2-methoxyphenyl-β-D-glucopyranoside (6). Conclusion Compound 1 was identified as a new alkaloid, named dendronobiline A1, while compound 2 was identified as a new phenylethanoid glycoside, named dendronobiside A1. Compounds 3—6 are isolated from the plant for the first time.

Dendrobium nobile Lindl.  /  alkaloid  /  glycoside  /  dendronobiline A1  /  dendronobiside A1  /  benzyl-β-D-glucoside  /  icariside D₂