ObjectiveTo systematically analyze the TIFY gene family members in Gastrodia elata f. glauca and explore their potential molecular mechanisms in the interaction between G. elata. f. glauca and Armillaria gallica. Methods Based on the whole-genome data of G. elata f. glauca, bioinformatics methods were used to analyze the system evolution, gene structures, and chromosome distribution. The gene expression characteristicswere detected by qRT-PCR, and the jasmonic acid (JA) content was detected by mass spectrometry. The correlation between gene expression and JA content was further analyzed. Results A total of 30 TIFY family members were identified in G. elata f. glauca, belonging to four subfamilies: TIFY, JAZ, PPD, and ZML. They have highly conserved protein motifs and similar exon/intron structures, and are unevenly distributed on seven chromosomes. The number of conserved homologous genes in G. elata f. glauca is more than that in Arabidopsis thaliana, indicating a specific expansion of the TIFY family. The cis-acting element analysis revealed that the promoter regions of the TIFY family members in G. elata f. glauca are enriched with a large number of hormone response, stress response, and growth and development-related elements. Transcriptome analysis showed that A. gallica infection induced differential expression of 19 TIFY family members in G. elata f. glauca. In the mother tubers, GeJAZ4-3, GeJAZ8, and GeJAZ1-1 were significantly upregulated, and the expression levels of multiple members were significantly positively correlated with JA content, especially the expression level of GeJAZ4-3. Conclusion The TIFY gene family in G. elata f. glauca shows specific expansion. GeJAZ4-3, as a key regulatory factor, mediates the JA signaling pathway to regulate the balance of the interaction between G. elata f. glauca and A. gallica, laying an important foundation for the analysis of the symbiotic mechanism of G. elata and facilitating disease-resistant breeding.

Gastrodia elata Bl.  /  Gastrodia elata Bl. f. glauca S. ChowTIFY gene family  /  bioinformatics analysis  /  jasmonic acid  /  expression pattern