Objective To investigate the secondary metabolites produced by marine-derived fungi Talaromyces sp. 13-z-02 and their anti-inflammatory activities were studied. Methods The isolation and purification of compounds were performed using silica gel and ODS column chromatography, semi-preparative HPLC. And their structures were elucidated by MS and NMR analyses. The isolated compounds were evaluated for their anti-inflammatory activity in zebrafish. Results One new isochromane and eleven known compounds were isolated and identified as 8-hydroxy-6-methoxy-7-methylisochromane (1) from the fermentation broth of this strain, bacillisporin B (2), macrosporusone D (3), bacillisporin F (4), 1-epi-bacillisporin F (5), macrosporusone E and 1-epi-macrosporusone E (6), bacillisporin A (7), xenoclauxin (8), Sch 725680 (9), aculene C (10), 6-ethyl-2-hydroxy-4-methoxy-3-methylbenzaldehyde (11), 6-ethyl-2,4-dihydroxy-3-methylbenzaldehyde (12). Compounds 1 and 12 significantly reduced the migration of immune cells with the same concentration of 20 μmol/L and exhibited anti-inflammatory activity. Conclusion Compound 1 is a new isochromane, designated as talaroslisochromane. Compounds 1 and 12 showed anti-inflammatory activity.

marine-derived fungi  /  Talaromyces sp.13-z-02  /  secondary metabolites  /  anti-inflammatory activity  /  talaroslisochromane  /  6-ethyl-2,4-dihydroxy-3-methylbenzaldehyde