Objective To screen high-efficiency phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of Fritillaria taipaiensis and provide data support for the development of microbial fertilizers. Methods Rhizosphere soil samples were collected from ten production areas. Strains of inorganic phosphate-solubilizing bacteria (IPSB) and organic phosphate-solubilizing bacteria (OPSB) were isolated and purified. Their phosphate-solubilizing capacities were evaluated qualitatively and quantitatively, and the strains were identified based on physiological and biochemical characteristics as well as 16S rDNA sequencing. The effects of selected strains were further verified using pot experiments. Results A total of 42 IPSB and 31 OPSB strains were isolated. Quantitative analysis showed that among the IPSB strains, WP2-2 exhibited the strongest solubilizing capacity, increasing available phosphorus by 231.30 mg/L, followed by WP7-2 (211.05 mg/L). Among the OPSB strains, YP3-1 showed the highest phosphorus solubilization (28.57 mg/L), followed by YP5-1 (22.30 mg/L). Strain WP2-2 was identified as Serratia plymuthica, WP7-2 as Bacillus cereus, and both YP3-1 and YP5-1 were also identified as B. cereus. Pot experiments revealed that inoculation with these PSB strains altered the total phosphorus content in bulbs and fibrous roots, and also influenced the total alkaloid content in the bulbs. Conclusion The strains WP2-2, WP7-2, YP3-1, and YP5-1 show great potential for developing specialized microbial fertilizers for the cultivation of F. taipaiensis.

Fritillaria taipaiensis P. Y. Li  /  phosphate-solubilizing bacteria  /  rhizosphere soil  /  separation and identification  /  inoculation effect