Objective To investigate the chemical constituents from the whole plant of Physalis angulata and their anti-inflammatory activities. Methods Various chromatographic techniques were employed for the isolation and purification of compounds, and their structures were elucidated by spectroscopic methods including NMR, MS, and UV. The cytotoxicity against mouse macrophage RAW264.7 cells was evaluated using the SRB assay, while the in vitro anti-inflammatory activity was determined via the Griess assay. Results Five compounds were isolated from the methanol extract of the whole plant of P. angulata and identified as 6- 5β,6β:14α,17:14β,26-triepoxy-13α,15β-dihydroxy-1-keto-11,15-cyclo-13,14-secoergostan-2-en-18,26-olide (1), physalin B (2), physalin F (3), physalin XI (4), and aspergillus triazolate A (5). Compounds 2—5 exhibited inhibitory effects on NO production in RAW264.7 cells, with IC₅₀ values ranging from (1.97 ± 0.12) to (6.73 ± 0.71) μmol/L. Conclusion Compound 1 is a new compound, named 5,6-epoxyphysalin IX. Compounds 4 and 5 showed significant anti-inflammatory activities.

Physalis L.  /  Physalis angulate L.  /  steroids  /  5,6-epoxyphysalin IX  /  physalin B  /  physalin XI  /  aspergillus triazolate A  /  anti-inflammatory activity