Objective To clone the full-length cDNA sequence of the CoMYB62 gene from Cornus officinalis, and analyze the structural characteristics, phylogenetic relationships, subcellular localization, and tissue expression patterns of its encoded protein, thereby providing a molecular basis for elucidating the potential roles of MYB transcription factors in the growth, development, and environmental adaptation of C. officinalis. Methods Based on transcriptome data of C. officinalis, the CoMYB62 gene sequence was screened and its full-length cDNA sequence was cloned using RT-PCR. Bioinformatics analyses were conducted to predict the physicochemical properties, conserved domains, secondary and tertiary structures, and phylogenetic relationships of the CoMYB62-encoded protein. A pCAMBIA1300-CoMYB62-GFP fusion expression vector was constructed and transiently expressed in tobacco leaves, and the subcellular localization of the CoMYB62 protein was observed using a laser confocal inverted microscope. In addition, quantitative real-time PCR (qRT-PCR) was performed to analyze the expression patterns of CoMYB62 in four tissues, including root, stem, leaf, and fruit. Results The full-length cDNA of the CoMYB62 gene was successfully cloned. Sequence analysis indicated that the encoded protein belonged to a typical R2R3-MYB transcription factor and contained a highly conserved DNA-binding domain at the N-terminus, which was predicted to be mainly localized in the nucleus. Phylogenetic analysis revealed that CoMYB62 shared high evolutionary conservation with MYB homologous proteins from various plant species. Laser confocal microscopy further confirmed that the CoMYB62 protein was localized in the nucleus. The qRT-PCR results showed that CoMYB62 was expressed in all examined tissues of C. officinalis, although the expression levels varied significantly among different tissues. Conclusion CoMYB62 is a conserved MYB transcription factor in C. officinalis with tissue-specific expression characteristics, which may participate in the regulation of plant growth, development, and stress responses to provide a foundation for further biological functional analysis of CoMYB62 in C. officinalis.

Cornus officinalis Sieb. et Zucc.  /  MYB transcription factor  /  gene cloning  /  expression analysis  /  subcellular localization