Objective To study the chemical constituents from the ethyl acetate extract of Alhagi sparsifolia Shap. Methods The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS column chromatography, and semi‑preparative HPLC, and their structures were identified by modern spectroscopic techniques. The anti‑cervical cancer activities of the compounds against human HeLa cells were evaluated by the MTT assay. Results Fifteen compounds were isolated from the ethyl acetate extract of A. sparsifolia and identified as 1-{1-[4-(4-hydroxyphenethoxy)phenethyl]-1H-pyrrol-2-l}ethan-1-one(1), 5,7-dihydroxy-6-methoxyflavone (2), callyspongidipeptide A (3), cyclo-(D-proline-D-valine) (4), 7-O-ethylguaiacyl glycerol (5), 7-O-ethylguaiacyl glycerol (6), isololiolide (7), N-phenethylacetamide (8), skullcapflavone II (9), 4-hydroxyphenethyl alcohol (10), N-phenethylformamide (11), 3′-methoxydaidzein (12), cyclo(alanine-proline) (13), N-(p-hydroxyphenethyl)formamide (14), and N-acetyltyramine (15). Conclusions Compound 1 is a new compound and named as alhagipyrrolizidine A. Compounds 1, 5 and 6 showed significant activity against human HeLa cells, with IC₅₀ values of 31.4, 26.6 and 37.3 μmol/L, respectively.

alhagipyrrolizidine A  /  cervical cancer  /  Alhagi sparsifolia Shap.  /  alhagipyrrolizidine A  /  7-O-ethylguaiacyl glycerol  /  7-O-ethylguaiacyl glycerol  /  skullcapflavone II