Objective To compare the contents of saponins, flavonoids and polysaccharides in the extracts of stems and leaves of Panax notoginseng from different sources and different processes (DRNJE) and to study their lipid-regulating effects. Methods The solvent gradient extraction method was used to remove protein by degreasing with petroleum ether, extracting flavonoids with ethyl acetate, extracting saponins with water-saturated n-butanol and adjusting the ratio of chloroform to n-butanol to 25∶4∶1. The contents of saponins, flavonoids and polysaccharides in the stem and leaf extracts of P. notoginseng were determined by ultraviolet spectrophotometer, and the contents of monomer saponins Rb₃, Rb₁ and Rc were simultaneously detected by HPLC. HepG2 cells induced by pancreatic lipase and oleic acid were used to study the lipid-regulating effect of DRNJE. Results After decolorization by macroporous resin/ion exchange, the contents of total saponins, monomer saponins and overall functional components of P. notoginseng stem and leaf extracts Y0729, J0801, J1020, Y1114, J1031 and R1001 were high. The inhibitory activity of lipase from high to low is: Y0729 > J1031 > J1020 > R1001 > Y1114 > J0801 > Q0901 > Q1001 > W1107 > W1101. According to the results of oil red O staining, DRNJE has lipid-regulating effect, among which Y0729, J1020, J1031 and R1001 have better effects. Conclusion After decolorization by macroporous resin/ion exchange, the extract of P. notoginseng stems and leaves has high content of saponins, flavonoids and polysaccharides, among which the extract of P. notoginseng stems and leaves with high saponin content has a significant effect on lipid regulation of HepG2 cells induced by lipase and oleic acid.

Panax notoginseng stem and leaf extract  /  saponins  /  flavonoids  /  polysaccharide  /  ginsenoside Rb₃  /  ginsenoside Rb₁  /  ginsenoside Rc  /  lipid regulation