Objective To clone the SmABCG34 gene from the Salvia miltiorrhiza genome and analyze its bioinformatics and expression characteristics. Methods The SmABCG34 gene was cloned from the S. miltiorrhiza genome, and the Conservative Domain and physicochemical properties of the gene and its encoded protein were analyzed by Bioinformatics Online Website. Quantitative real-time PCR (qRT-PCR) was used to detect the tissue expression specificity of SmABCG34 gene and its response to stress induced by abscisic acid (ABA), trans-zeatin (tZ), and polyethylene glycol-6000 (PEG-6000). Results The CDS of the SmABCG34 gene is 4 347 bp in size and encodes 1 448 amino acids; This protein contains a typical NBD-TMD-NBD-TMD structure and belongs to the full-molecule transporter of the ABCG subfamily; subcellular localization shows that this protein was localizted to the cell membrane. Phylogenetic analysis showed that SmABCG34 was closely related to S. hispanica, with a protein homology of up to 91.23 %. The expression pattern analysis revealed that the SmABCG34 gene was expressed in the roots, stems, leaves and flowers of S. miltiorrhiza with the highest expression in the flowers, followed by the leaves. After treatment with ABA, tZ, and PEG-6000, compared to 0 h, the expression of the SmABCG34 gene was significantly upregulated at 12 h. Conclusion The SmABCG34 gene belongs to the ABCG subfamily and shows a significant response to hormone and stress treatments, providing a scientific basis for further exploration of the function of the SmABCG34 gene.

Salvia miltiorrhiza Bge.  /  SmABCG34 gene  /  gene cloning  /  bioinformatics analysis  /  expression analysis