Objective To investigate the chemical constituents from Sanghuangporus vaninii and their activity against tumor necrosis factor-α (TNF-α)-induced human rheumatoid arthritis fibroblast synovial cells (MH7A). Methods Isolation and purification were carried out by using various separation techniques such as silica gel, ODS column chromatography, and semi-preparative high-performance liquid chromatography. The structures of isolated compounds were elucidated through comprehensive spectroscopic analyses, such as UV, IR, HRESIMS, NMR, carbon spectrum calculation and ECD. Meanwhile, the activity of sesquiterpene compounds in inhibiting the proliferation of MH7A cells induced by TNF-α was evaluated by the CCK-8 method and the levels of inflammatory cytokines IL-6 and IL-1β were detected by ELISA. Results A total of nine compounds were isolated from 95% ethanol extract of S. vaninii and were identified as (4S,5S,6R)-2-methyl-5,6-dihydroxy-[R-6-(4-hydroxymethyl)-3-cyclohexene]-furan-2(5H)-one (1), phellilane D (2), phellilane C (3), (＋)-γ-ionylideneacetic acid (4), elgonene A (5), (3E)-4-(3,4-dihydroxyphenyl)-but-3-en-2-one (6), 3,4-dihydroxybenzoate (7), (E)-4-(4-hydroxyphenyl)but-3-en-2-one (8), protocatechualdehyde (9), respectively. The bioactivity results showed that compounds1 ,3 and4 significantly inhibited the proliferation of TNF-α-induced MH7A cells within 20 μmol/L, and the inhibition rates were (38.9 ± 1.32)%、(46.21 ± 0.86)% and (36.59 ± 1.51)% at 20 μmol/L, respectively. Furthermore, the ELISA results showed they effectively reduced the production of IL-6 and IL-1β on TNF-α-induced MH7A cells. Conclusion Compounds1 —5 were identified as bisabolane type sesquiterpenoids and6 —9 were identified as phenols. Compound1 was a new sesquiterpenoid and named as phellilane Q, Compound7 was first isolated from S. vaninii. Compounds1 ,3 and4 significantly suppressed the proliferation of TNF-α-induced MH7A cells and demonstrated anti-inflammatory effects.

Sanghuangporus vaninii (Ljub) L. W. Zhou et Y. C. Dai  /  sesquiterpenoids  /  phenols  /  anti-rheumatoid arthritis  /  phellilane Q  /  phellilane C  /  (+)-γ-ionylideneacetic acid