In recent years, protoplast technology has widely applied in the fields of plant genetic transformation, breeding, and plant regeneration. Related research on model plants (such as tobacco and Arabidopsis thaliana) has been in-depth, and its application in crops like forage grass, rice, and tomatoes has also become more and more mature. With the development of the economy and the increase in health demands, the market demand for medicinal plants has been continuously growing. However, the research on their breeding and genetic transformation is still insufficient, and protoplast technology can just provide a new path for this. This paper focuses on the preparation and isolation techniques of protoplasts of medicinal plants and their application scenarios, reviews their research status and challenges, and provides a reference for in-depth research on protoplasts of medicinal plants in fields such as genetic transformation, variety improvement, and plant regeneration.

medicinal plants  /  protoplasts  /  isolation  /  transient transformation  /  regenerated plant  /  gene editing