Objective To mine CYP gene family members in Coptis chinensis based on genomic data and analyze the evolutionary specificity of CYP719 genes. Methods The CYP gene family members of C. chinensis were identified based on homologous CYP gene sequence from both Arabidopsis thaliana and Oryza sativa. Analyses of the physicochemical properties of proteins, system classification, gene structure, expression profile and cis-acting elements of the identified CYP genes were further conducted by bioinformatics. The CYP719 family members in C. chinensis were identified based on the 23 reported CYP719 genes in other plants, and their collinearity analysis was performed through homologous sequence alignment. Results A total of 308 CYP genes were identified in the C. chinensis genomic data, belonging to 45 families in nine family clans. The range of amino acid numbers for encoded proteins is from 103 to 2 008 aa, with a relative molecular mass ranging from 11 010 to 230 370, and an isoelectric point ranging from 4.85 to 9.79. The number of CYP gene exons in C. chinensis varies between 1 and 38, with a wide range of variation. A total of 308 CYP genes were unevenly distributed on nine chromosomes of C. chinensis and mainly expanded through dispersed duplication（DSD） and proximal duplication（PD）. The expression of CYP genes was tissue-specific, and nine genes were significantly upregulated in rhizomes and fibrous roots. In the promoter regions of these CYP genes, stress response elements were predicted to be the most frequently occurring cis-acting elements. Through genomic collinear analyses between different plants, the C. chinensis CYP719 genes have homologous ones in Aquilegia coerulea, Corydalis tomentella, Papaver somniferum, and Aristolochia debilis, but not in Arabidopsis thaliana, Glycine max, Prunus persica, Chrysanthemum nankingense, Vitis vinifera, Aconitum vilmorinianum, O. sativa, and Amborella trichopoda. Conclusion C. chinensis has a rich CYP supergene family, in which CYP719 gene shows branch-specific evolution, laying the foundation for analyzing the functions of this type of gene.

Coptis chinensis Franch.  /  cytochrome P450  /  bioinformatics analysis  /  expression analysis  /  dispersed duplication  /  proximal duplication