Objective Based on the mathematical model of mass transfer, this study aimed to investigate the existence state and transfer principle of chlorogenic acid in the production process of Reduning Injection (RI, 热毒宁注射液) were investigated. Methods Using chlorogenic acid as the detection index, a nanofiltration mass transfer model was established., and choosing the monomer molecular state as the reference, calculated the existence state of chlorogenic acid in the intermediate solution of RI, analyzed the correlation between the change of chlorogenic acid transfer rate and the existence state of chlorogenic acid in the process of production of RI, and explored the intrinsic causes of the difference in the transfer of phenolic acids during the RI concentration process. Results The regression coefficients between the mass transfer coefficients of chlorogenic acid and the power value of solute concentration in the extract solution and the RI intermediate solution were all greater than 0.90, and the nanofiltration mass transfer model was established. chlorogenic acid showed a positive correlation between the ratio of molecular states and the concentration transfer rate in aqueous and ethanol solutions of RI intermediates, and the same trend was observed in solutions of Jinyinhua (Lonicerae Japonicae Flos) and Qinghao (Artemisiae Annuae Herba) intermediates, suggesting that the state of existence of the constituents is the intrinsic cause of the differences in phenolic acid transfer during RI concentration process. In addition, chlorogenic acid was more stable in the molecular state than in the ionic state in the same solution system, and more stable in the ethanol solution than in the aqueous solution in different solvent systems. Conclusion A quantitative calculation method for the molecular state of chlorogenic acid in the intermediate of RI has been constructed, and the positive correlation between its existence state and mass transfer has been initially elucidated, which can provide support for the standardized control of the production process of the preparation.

chlorogenic acid  /  Reduning Injection  /  nanofiltration  /  mass transfer model  /  existence state  /  mass transfer principle