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To study how lipid bilayer fluidity modulates the interaction between β1 integrin and CD40L, as well as the formation of CD40L-mediated tumor cell contact interfaces.
Supported lipid bilayers (SLB) with different fluidities were prepared through adjusting the 1, 2-dioleoyl-sn-glycero-3-[N-(5-amino-1-carboxypentyl) iminodiacetic acid] succinyl nickel salt (DGS-NTA) content. The functionalization of lipid bilayers was achieved by anchoring fluorescently labeled CD40L molecules onto the membrane surface. The contact interface formation of PC9 cells on the functionalized lipid bilayers was observed through confocal fluorescence imaging and fluorescence recovery after photobleaching (FRAP) experiments, and data of two dimensional (2D) reaction kinetics of β1 integrin and CD40L were extracted from Zhu-Golan plots.
The diffusion coefficient of molecules in lipid bilayer was negatively correlated with DGS-NTA content. High fluidity of lipid bilayer promoted CD40L accumulation at cell contact interface and expanded the cell contact area. The 2D dissociation constants (2D Kd) of β1 integrin-CD40L complexes were approximately 13, 31 and 65 molecules/μm2 for the three lipid bilayers with high, moderate and low fluidities, respectively.
High fluidity of lipid bilayers significantly facilitates diffusion and aggregation of CD40L to the cell contact interface, thus enhancing β1 integrin-CD40L interaction and the stability of cell contact interfaces.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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