In-situ leaching uranium has become one of the important techniques in uranium mining and metallurgy of China after more than 40 years of experimental research and industrial application. However, the radioactive wastewater, radioactive waste gas and radioactive solid wastewater produced by long-term in-situ leaching uranium will have impact of different extent on the ecological environment around the mine, which pose adverse effect on public health and environmental safety. In order to understand the radiation environment of in-situ leaching uranium mine, taking an in-situ leaching uranium mine in Xinjiang as the investigation object, radiation environment investigation and research during production and operation period of mine were carried out on the basis of the site investigation and sample analysis. The results show that the radioactivity levels of monitoring media such as ambient air, terrestrial gamma, surface water, groundwater, soil, biological samples, and radon exhalation rate around the in-situ leaching uranium mine are basically within the radioactive background level range of Xinjiang region or the applied standard limits, besides the radiation environmental quality meet the corresponding requirements and the radiation environmental risk is controllable. In the future production and operation period of in-situ leaching mine, mining enterprises should strengthen the awareness of environmental protection, through establishing and improving environmental management and monitoring plans, formulating scientific and effective radiation protection measures, strengthening environmental monitoring and emergency management and other measures to ensure the impact of mining activities on the environment is minimized, public health and environmental safety is guaranteed and development of in-situ leaching uranium mining is further improved.