The use of isotopes are becoming more and more common in imaging, diagnosis and treatment, which bring potential radiation hazards to radiation workers and the public. By exploring the radiation protection measures of the nuclear medicine discipline, based on the monitoring and analysis of the current situation of the nuclear medicine discipline in a hospital and the field investigation, the radiation protection effect of the radioisotope application site in the hospital was analyzed and evaluated. The results show that the radiation level around the radiation workplace of the nuclear medicine department is lower than the dose limit specified in the standard, and the estimated annual effective dose is less than the management target value, which meet the relevant standards of radiation protection. The shielding effect of the shielding body in various places of the hospital is good, and the shielding of the nuclear medicine institute is feasible.