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The objective of this study was to isolate and characterize a bovine-yak origin group A rotavirus with the G8P［11］ genotype and to analyze the genetic evolutionary features of its genome. Fecal samples from diarrheic calves at a dairy farm in Sihong， Jiangsu Province， were screened for six diarrhea-associated viruses. Rotavirus （RV）-positive samples were isolated and identified using RT-PCR， indirect immunofluorescence assay （IFA）， dsRNA-PAGE， and electron microscopy. The growth kinetics and cellular tropism of the isolated strains were characterized， and their cross-species transmission potential was evaluated. Additionally， whole-genome sequencing was performed on the isolates， followed by phylogenetic tree construction to determine their G/P genotypes. A bovine rotavirus strain was successfully isolated. Inoculation onto MA104 cells induced typical cytopathic effects， including cell rounding， shrinkage， detachment， and fragmentation. The virus reacted with VP6-specific monoclonal antibodies， exhibited a 4∶2∶3∶2 genomic RNA electrophoretic pattern， and displayed characteristic wheel-shaped virions approximately 70 nm in diameter. This strain was designated JSSH2024. A one-step growth curve revealed peak viral titers （TCID50） as 105.75·mL-1 in MA104 cells at 18 hours post-infection. In vitro infection assays demonstrated the virus's ability to replicate in MA104， IPEC-J2， HT-29 and HCT-8 cell lines， indicating potential for cross-species transmission. Whole-genome sequencing and phylogenetic analysis identified its genotype as G8-P［11］-I2-R2-C2-M2-A13-N2-T6-E2-H3. Eight gene segments clustered closely with bovine rotaviruses， while three segments showed closer genetic affinity to yak rotaviruses， suggesting its origin as a bovine-yak reassortant virus. This study reports the first G8P［11］ bovine-yak reassortant bovine rotavirus （BoRV） in China， providing critical insights for epidemiological surveillance and vaccine development.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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