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The objective of this study was to investigate the imprinting status and to search for potential DNA differentially methylated regions （DMRs） of the DLK1-DIO3 domain in bovine placentas. In this study， whole genome sequencing （WGS） was performed on 3 bovine placentas and their parents to mining the informative single nucleotide polymorphisms （SNPs）. The imprinting status of genes in the DLK1-DIO3 domain were determined by the combined analysis of WGS and transcriptome sequencing （RNA-seq） results. The DMRs were identified by combination of WGS and whole genome bisulfite sequencing （WGBS） data. WGS and RNA-seq results showed that MEG3， MEG8 and MEG9 genes were maternally expressed imprinted genes and DLK1， RTL1 and DIO3 genes were paternally expressed imprinted genes. The WGBS data analysis showed that 4 DMRs， Dlk1-DMR， IG-DMR， Gtl2-DMR and Meg8-DMR were conserved in human and mouse， and 5 new DMRs were identified， DLK1-DMR0 and DLK1-DMR1 located upstream of the DLK1 gene， MEG9-DMR in MEG9 promoter region， and DIO3-DMR1 and DIO3-DMR2 located upstream of DIO3 genes， respectively. The results indicate that the imprinted status and differentially methylated regions of bovine DLK1-DIO3 imprinted region are highly conserved to human and mouse. In addition， 5 potential DMRs were identified in bovine DLK1-DIO3 region， and their regulating mechanism on the DLK1-DIO3 imprinted region needs to be further investigated.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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