Full
Outline
This study aimed to investigate the effects of gut microbiota on the production performance of aged laying hens during the late laying period by altering their intestinal microbiota structure through fecal microbiota transplantation （FMT）. Forty 28-week-old and 250 68-week-old hens were observed for 7 weeks to record their laying rate. The hens were divided into three groups based on the laying rate （LR） and age which recorded previously： 35-week-old high-yielding hens （YH， LR>90%， 37 hens）， 75-week-old high-yielding hens （OH， LR>90%， 76 hens）， and 75-week-old low-yielding hens （OL， 30%<LR<60%，104 hens）. FMT was then performed at the 8th week for 4 weeks to remodel the gut microbiota of aged hens： aged high-yielding hens received fecal microbiota suspension from young high-yielding hens （OHFMT-YH group）， while aged low-yielding hens received fecal microbiota suspension from aged high-yielding hens （OLFMT-OH group）. Control groups （OH and OL） were established， with each group comprising 5 replicates of 6 hens. The results were showed as follows： Compared to the OH group， aged high-yielding hens transplanted with microbiota from young high-yielding hens （OHFMT-YH group） exhibited significant decreases （P<0.05） in laying rate， eggshell strength， eggshell thickness， serum sIgA and IL-10 levels， jejunal villus height， mucosal layer thickness， jejunal FOXP3 and ZO-1 mRNA expression， acetate content， and Bacteroidetes abundance. Conversely， yolk weight， serum IL-1β， IL-17A， and IL-6 levels， cecal mucosal layer and intestinal wall thickness， and Firmicutes abundance significantly increased （P<0.05）， the number of OTUs decreased， and the structure of the flora was obviously separated. In the OLFMT-OH group， compared to the OL group， aged low-yielding hens transplanted with microbiota from aged high-yielding hens showed significant increases （P<0.05） in laying rate， eggshell strength， serum IL-10 levels， jejunal villus height and mucosal layer thickness， FOXP3 and ZO-1 mRNA expression， goblet cell count， cecal mucosal layer thickness， acetate content， and Bacteroidetes abundance. However， egg weight， egg longitudinal diameter， yolk weight， serum IL-1β levels， cecal intestinal wall thickness， jejunal STAT3 mRNA expression， and Firmicutes abundance significantly decreased （P<0.05）. β-diversity showed that the structure of the microbiota had changed. The LEfSe analysis revealed that Firmicutes showed the greatest contribution to the differences in both the OHFMT-YH and OL groups， while Bacteroidetes were the most significant contributors to the disparities in the OH and OLFMT-OH groups. In conclusion， FMT from young high-yielding hens to aged high-yielding hens increased Firmicutes abundance in the gut but reduced laying performance. Conversely， enhancing Bacteroidetes abundance in aged low-yielding hens through FMT from aged high-yielding hens improved gut microbiota structure， intestinal health， and laying performance. These findings suggest that modulating gut microbiota composition via FMT may serve as a potential strategy to enhance productivity in aged laying hens during the late laying period.
162
75
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
