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Recent Research Progress on the Sources and Detection Methods of β-lactamase in Milk
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Wenjie CHENG1, 2, 3, Nan ZHENG1, 2, Yankun ZHAO4, Jiaqi WANG1, 2, Lu MENG1, 2
Acta Veterinaria et Zootechnica Sinica | 2026, 57(2) : 638 - 650
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Acta Veterinaria et Zootechnica Sinica | 2026, 57(2): 638-650
• REVIEW •
Recent Research Progress on the Sources and Detection Methods of β-lactamase in Milk
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Wenjie CHENG1, 2, 3, Nan ZHENG1, 2, Yankun ZHAO4, Jiaqi WANG1, 2, Lu MENG1, 2
Affiliations
  • 1.Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs （Beijing），Institute of Animal Sciences，Chinese Academy of Agricultural Sciences，Beijing 100193，China
  • 2.Key Laboratory of Dairy Quality Digital Intelligence Monitoring Technology，State Administration for Market Regulation，Beijing 100193，China
  • 3.School of Food Science and Engineering，Qingdao Agricultural University，Qingdao 266109，China
  • 4.Xinjiang Academy of Agricultural Sciences，Urumqi 830091，China
Published: 2026-02-23 doi: 10.11843/j.issn.0366-6964.2026.02.006
Outline
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β-lactamase is an enzyme capable of hydrolyzing β-lactam antibiotics， and its residues in milk are increasingly concerning. The endogenous factors related to β-lactamase residues in milk are associated with the health status of the cows themselves， while exogenous factors mainly involve illegal human addition. The residues of β-lactamase may cause various health risks， including the accumulation of cytotoxic degradation products， the failure of antibiotics， and drug resistance development in humans. Currently， detection methods for β-lactamase residues in milk include microbial methods， iodometry， acidity meter method， instrumental detection method， and rapid detection method， etc.， but all these methods have limitations. This article reviews the biological characteristics， sources， and recent research progress on detection methods of β-lactamase in bovine milk. Also discusses the limitations of current detection methods and provides an outlook on future management measures and emerging detection technologies. The aim is to provide scientific references for detection personnel and researchers， ultimately enhancing dairy product safety.

milk  /  β-lactamase  /  β-lactamase residues  /  detection method
程文杰, 郑楠, 赵艳坤, 王加启, 孟璐. 牛乳中β-内酰胺酶的来源与检测方法最新研究进展. 畜牧兽医学报, 2026 , 57 (2) : 638 -650 . DOI: 10.11843/j.issn.0366-6964.2026.02.006
Wenjie CHENG, Nan ZHENG, Yankun ZHAO, Jiaqi WANG, Lu MENG. Recent Research Progress on the Sources and Detection Methods of β-lactamase in Milk[J]. Acta Veterinaria et Zootechnica Sinica, 2026 , 57 (2) : 638 -650 . DOI: 10.11843/j.issn.0366-6964.2026.02.006
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Year 2026 volume 57 Issue 2
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Article Info
doi: 10.11843/j.issn.0366-6964.2026.02.006
  • Receive Date：2025-02-06
  • Online Date：2026-09-16
  • Published：2026-02-23
Article Data
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History
  • Received：2025-02-06
Funding
Affiliations
    1.Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs （Beijing），Institute of Animal Sciences，Chinese Academy of Agricultural Sciences，Beijing 100193，China
    2.Key Laboratory of Dairy Quality Digital Intelligence Monitoring Technology，State Administration for Market Regulation，Beijing 100193，China
    3.School of Food Science and Engineering，Qingdao Agricultural University，Qingdao 266109，China
    4.Xinjiang Academy of Agricultural Sciences，Urumqi 830091，China

Corresponding:

MENG Lu， E-mail：
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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