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Porcine epidemic diarrhea virus （PEDV） is a coronavirus that causes acute diarrhea， vomiting， and dehydration in piglets， among other clinical signs， and has caused severe economic losses to the global swine farming industry. More and more studies have reported that long non-coding RNAs （lncRNAs） play important regulatory roles in porcine coronaviruses， however， the role and mechanism of lncRNA 663 in PEDV infection remain unclear. Based on prior transcriptome sequencing， we identified a co-expression pattern between lncRNA 663 and the tight junction protein CLDN10. This study aimed to explore their roles and regulatory relationship during PEDV infection. The qRT-PCR and Western blot results showed that the expression levels of lncRNA 663 and CLDN10 were up-regulated after PEDV infection of IPEC-J2 cells （P<0.05）， DSS treatment significantly reduced the expression levels of lncRNA 663 and CLDN10 （P<0.05）. Subcellular localization prediction revealed that lncRNA 663 was mainly localized in the cytoplasm， and RPISeq prediction revealed that lncRNA663 and CLDN10 were highly interoperable （SVM： 0.98， RF： 0.8）. Functional experiments demonstrated that siRNA-mediated knockdown of lncRNA 663 led to a significant decrease in both CLDN10 expression and PEDV replication （P<0.05）. Furthermore， structure prediction using AlphaFold3 and ChimeraX revealed potential hydrogen bond interactions between CLDN10 and the PEDV spike （S） protein， suggesting a role in viral entry or infection. Collectively， these results suggest that lncRNA 663 may promote PEDV replication by regulating CLDN10 expression. These findings expand our understanding of PEDV-host interactions and provide a theoretical basis for future antiviral strategies targeting host non-coding RNAs.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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