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This experiment aimed to investigate the effects of ZNF697 on the proliferation and differentiation of porcine skeletal muscle satellite cells （MuSCs）. In this experiment， porcine MuSCs were used as the research object. Flow cytometry， Real-time quantitative PCR， Western blot and immunofluorescence staining were employed to detect the effects of ZNF697 on the proliferation and differentiation of porcine MuSCs. The following results were obtained： 1） The expression of ZNF697 mRNA dynamically changes with the proliferation of porcine MuSCs. Interfering with ZNF697 inhibited the mRNA expression of Cyclin E （P<0.01） and Cyclin D （P<0.05） and the protein expression of Cyclin B （P<0.05）， promoted the mRNA expression of p21 （P<0.01）. Overexpression of ZNF697 showed the opposite results. 2） During the myogenic differentiation of porcine MuSCs， the expression of ZNF697 gradually increased. Interfering with ZNF697 significantly reduced the myogenic differentiation index （P<0.05） and inhibited the mRNA expression of MyoG （P<0.01）， the mRNA and protein expression of MyHC （P<0.05） and MyoD （P<0.01）. Overexpression of ZNF697 showed the opposite results. The results show that ZNF697 is a promoter of proliferation and myogenic differentiation in porcine MuSCs， which will provide a breeding target for high-lean-meat pig breeds.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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