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Outline
This study aimed to investigate preventive effects of aspirin eugenol ester （AEE） on oxidative stress and inflammatory response in the ovaries of hens with fatty liver hemorrhagic syndrome （FLHS）. A total of sixty-five 160-day-old Hy-Line Brown laying hens were randomly assigned to five groups， each containing 13 hens. The control group （Control） was fed a basal diet， while the model group （high-energy low-protein diet， HELP） and low-， medium-， and high-dose AEE treatment groups （AEE-L， AEE-M， AEE-H） were all fed a high-energy low-protein diet supplemented with 0， 25， 50， and 100 mg·kg-1 aspirin eugenol ester， respectively. The results showed that， compared with the control group， the ovaries of laying hens in the HELP group exhibited distinct lipid vacuoles of varying sizes. In contrast， the number of lipid vacuoles in the ovarian tissue was significantly reduced in the AEE-treated groups compared with the HELP group. Compared with the control group， the activities of SOD， GSH， and CAT in the ovaries of hens in the HELP group were significantly decreased， while the level of MDA was significantly increased （P<0.05）. In contrast， AEE treatment groups demonstrated significantly enhanced SOD， CAT， and GSH activities and reduced MDA levels and significantly upregulated SOD and CAT mRNA transcription compared with the HELP group （P<0.05）. Compared with the Control group， the expression levels of antioxidant-related genes and proteins Nrf2， NQO1， and HO-1 were significantly decreased in the ovaries of hens in the HELP group， while the expression of Keap1 was significantly increased （P<0.05）. In contrast， the AEE-treated groups showed significantly increased expression of Nrf2， NQO1， and HO-1 and decreased expression of Keap1 compared with the HELP group （P<0.05）. Compared with the control group， the mRNA transcription levels of inflammatory cytokines IL-1β， IL-6， and TNF-α were significantly increased in the ovaries of hens in the HELP group. In addition， the expression levels of inflammation-related genes and proteins-including TLR4， NF-κB， and p-p65 were also significantly elevated （P<0.05）. In contrast， AEE treatment groups exhibited significantly reduced expression of IL-1β， IL-6， TNF-α， TLR4 and NF-κB genes， along with significantly decreased p-p65 protein expression compared to the HELP group （P<0.05）. In summary， fatty liver hemorrhagic syndrome in laying hens can induce ovarian oxidative stress and inflammatory responses. Supplementation with varying doses of AEE demonstrated preventive effects against ovarian damage in laying hens with fatty liver hemorrhagic syndrome.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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