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This study adopted an IF feeding method to investigate the effects and mechanisms of this dietary approach on ovarian function in mice， aiming to provide a theoretical basis for livestock reproduction management. In this study， 8-week-old female C57BL/6J mice with similar body weights were randomly divided into two groups： one group served as the normal diet control group （ND）， while the other group was subjected to a cyclic pattern of “1 day of restricted feeding （70% feed intake）-1 day of free feeding” to establish an intermittent mild fasting （IMF） mouse model. The growth performance of the mice was measured， and ovarian function as well as the expression of autophagy-related proteins were detected through Western blot （WB） and immunohistochemistry experiments. The results showed that IMF could significantly reduce the feed-to-gain ratio （82.85±2.68 & 93.02±7.40） and abdominal fat index （1.49±0.11 & 1.72±0.19）. At the same time， the autophagy markers LC3Ⅱ/LC3Ⅰ（1.06±0.12 & 0.89±0.02） and reproductive function proteins BMP-15 （1.05±0.08 & 0.75±0.10） and GDF-9 （0.53±0.02 & 0.44±0.02） were up-regulated. The mechanism study showed that IMF activated autophagy by inhibiting mTOR pathway （p-mTOR/mTOR 0.82±0.10 & 0.97±0.05）， and then improved ovarian function. This study is the first to elucidate the mechanism by which IMF enhances ovarian function through autophagy activation， offering a potential non-pharmaceutical strategy for developing reproductive regulation techniques in livestock.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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