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This work aimed to prepare nanosilver and evaluate its therapeutic effect on methicillin-resistant Staphylococcus aureus infections in canine skin wounds. The nanosilver was prepared by biosynthesis method using Ganoderma lucidum extracts （40% polysaccharide content） as reducing agent and polyvinylpyrrolidone as stabilizing agent， followed by characterization using ultraviolet visible absorption spectroscopy， transmission electron microscopy and dynamic light scattering. Subsequently， the invitro antimicrobial activity of nanosilver was evaluated by determining the minimum inhibitory concentration. Finally， 12 Beagles weighing about 10 kg were randomly divided into 3 groups of 4 dogs each， namely， saline control group， enrofloxacin positive control group and nanosilver group. After establishing the model of wound infection with methicillin-resistant Staphylococcus aureus， wound healing， bacterial load， histopathological morphology， collagen fibers and angiogenesis， and macrophage polarization levels were tested and analyzed after 12 consecutive days of treatment. The results showed that the prepared nanosilver had a maximum absorption wavelength at 433 nm， and were spherical and uniformly distributed， with a particle size of （20.01±6.99） nm and zeta potential of （-2.56±0.32） mV. Nanosilver exhibited good antibacterial activity against common skin infection causative organisms containing methicillin-resistant Staphylococcus aureus， with a minimum inhibitory concentration of 16-32 μg·mL-1. The results of in vivo experiment showed that nanosilver could effectively reduce the bacterial load of wound tissues， promote collagen deposition and angiogenesis， and improve the immune microenvironment of wounds， which in turn promoted the healing of infected wounds. The results suggested that the prepared nanosilver can effectively treat methicillin-resistant Staphylococcus aureus induced canine skin wounds infections， laying a material and practical foundation for its translation and application to the clinical treatment of pet skin infections.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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