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Rabbit coccidiosis is a worldwide-distributed parasitic protozoal disease， which has caused huge economic losses to rabbit industry. Traditional methods of anticoccidial treatment result in drug resistance and drug residue， vaccination is a promising alternative. The rEiAMA1， rEiIMP1， EmGAM56， and EmROP17 were respectively displayed on the surface of B. subtilis spores by using a coat protein （CotB） as an anchor. Then the recombinant spores were used to oral immunize rabbits， the protective effects of B. subtilis-rEiAMA1， -rEiIMP1， and -rEiAMA1+rEiIMP1 against Eimeria intestinalis were evaluated； and the protective effects of B. subtilis-EmGAM56， -EmROP17， and -EmGAM56+ EmROP17 against Eimeria magna were evaluated. The results showed that B. subtilis-rEiAMA1 immunized rabbits displayed better anti-E. intestinalis protective effect， the oocyst output and lesion score were significantly decreased （P<0.05）， the oocyst output decrease ratio was 59.91%. B. subtilis-EmGAM56 immunized rabbits displayed better anti-E. magna protective effect， the oocyst output was significantly decreased （P<0.05）， the oocyst output decrease ratio was 68.54%. Moreover， the immunized rabbits showed significantly increased specific serum IgG levels and specific IgA levels （P<0.05）. The four recombinant B. subtilis spores can stimulate the body to produce specific intestinal and systemic humoral immune responses. B. subtilis-rEiAMA1 and B. subtilis-EmGAM56 showed better immunoprotection against E. intestinalis and E. magna， respectively. Therefore， B. subtilis is a potential live vector for rabbit coccidiosis vaccines.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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