Full
Outline
Cyclophosphamide （CTX） is a widely used chemotherapeutic agent for treating canine tumors； however， its potent immunosuppressive effects can impair immune function and disrupt gut microbiota homeostasis， increasing the risk of secondary complications. Lactobacillus murinus （L. murinus） has been reported to modulate immune cell activity and protect the gastrointestinal tract， highlighting its potential as a functional food additive. In this study， a probiotic strain with optimal characteristics， L. murinus ZNL-13， was isolated from the fresh feces of healthy dogs. The aim was to evaluate the effects of this canine-derived strain on CTX-induced immunosuppression and gut microbiota dysbiosis in dogs. Serum immune parameters and fecal 16S rRNA sequencing were used for analysis. Results showed that prophylactic administration of L. murinus ZNL-13 significantly improved CTX-induced reductions in white blood cells and lymphocytes （P<0.05）， alleviated liver and kidney injury， reduced oxidative stress （P<0.05）， and enhanced intestinal barrier function by lowering serum levels of DAO and D-lactic acid （P<0.01）. In addition， L. murinus ZNL-13 mitigated the CTX-induced decline in serum IgG and key immune cytokines （P<0.05）， increased the abundance of beneficial gut bacteria， and restored microbial homeostasis. Meanwhile， the levels of beneficial bacteria such as Blautia_A， Holdemanella， and Peptacetobacter were increased， contributing to the restoration of gut microbiota homeostasis. In conclusion， L. murinus ZNL-13 effectively counteracts CTX-induced immunosuppression， oxidative stress， and gut barrier dysfunction， offering a promising strategy for the development of novel functional food additives to support immune health in dogs.
136
59
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
