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Effect of circPRKAA2 on Milk Fat Synthesis in Dairy Cows via the miR-22-3p/SLC27A1 Axis
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Chuanchuan WANG1, 2, Yafei CHEN1, 2, Jia TIAN3, Xiaofang FENG4, Lijia TONG1, 2, Hua WANG1, 2, Lin XUE1, 2, Yaling GU1, 2, Juan ZHANG1, 2
Acta Veterinaria et Zootechnica Sinica | 2026, 57(2) : 740 - 752
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Acta Veterinaria et Zootechnica Sinica | 2026, 57(2): 740-752
• ANIMAL GENETICS AND BREEDING •
Effect of circPRKAA2 on Milk Fat Synthesis in Dairy Cows via the miR-22-3p/SLC27A1 Axis
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Chuanchuan WANG1, 2, Yafei CHEN1, 2, Jia TIAN3, Xiaofang FENG4, Lijia TONG1, 2, Hua WANG1, 2, Lin XUE1, 2, Yaling GU1, 2, Juan ZHANG1, 2
Affiliations
  • 1.College of Animal Science and Technology，Ningxia University，Yinchuan 750021，China
  • 2.Ningxia Hui Autonomous Region Key Laboratory of Molecular Cell Breeding，Yinchuan 750021，China
  • 3.Ningxia Hui Autonomous Region Animal Husbandry Workstation，Yinchuan 750021，China
  • 4.College of Life Sciences and Engineering，Northwest Minzu University，Lanzhou 730030，China
Published: 2026-02-23 doi: 10.11843/j.issn.0366-6964.2026.02.015
Outline
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The aim of this study was to explore the impact of molecular networks constructed by DE-circRNAs related to milk fat synthesis on milk fat synthesis in dairy cows through in-depth analysis. In this study， we used the circRNA library and transcriptome sequencing data of Holstein dairy cows with high and low milk fat percentage constructed previously to screen and identify DE-circ RNAs（circPRKAA2） related to milk fat synthesis， and further predicted their ceRNA regulatory network using Targetscan and miRanda software. Real-time fluorescence quantitative PCR， RNA pull down assay and dual luciferase reporter assay were used to verify the targeting relationship. Oil red O and BODIPY staining were used to investigate the effect of target miRNA on lipid droplet secretion in BMECs. The results indicated that the regulatory relationship of the ceRNA （circPRKAA2/miR-22-3p/SLC27A1） network was successfully predicted and verified， and 9 miRNAs and 10 candidate mRNAs were predicted， and it was clarified that circPRKAA2 and miR-22-3p were mainly distributed in the cytoplasm of BMECs. circPRKAA2 negatively regulated miR-22-3p during the process of milk fat synthesis in BMECs. miR-22-3p inhibited the secretion of lipid droplets， the production of triglycerides and cholesterol in BMECs， and negatively regulated SLC27A1. In conclusion， this study analyzed and verified the targeting relationship of circPRKAA2/miR-22-3p/SLC27A1 and its regulatory effect on milk fat synthesis， with the hope of providing new insights for improving milk quality.

Holstein cow  /  SLC27A1  /  miR-22-3p  /  circPRKAA2  /  milk fat
王川川, 陈亚飞, 田佳, 冯小芳, 佟丽佳, 王华, 雪林, 顾亚玲, 张娟. circPRKAA2通过调控miR-22-3p/SLC27A1轴对奶牛乳脂合成的影响. 畜牧兽医学报, 2026 , 57 (2) : 740 -752 . DOI: 10.11843/j.issn.0366-6964.2026.02.015
Chuanchuan WANG, Yafei CHEN, Jia TIAN, Xiaofang FENG, Lijia TONG, Hua WANG, Lin XUE, Yaling GU, Juan ZHANG. Effect of circPRKAA2 on Milk Fat Synthesis in Dairy Cows via the miR-22-3p/SLC27A1 Axis[J]. Acta Veterinaria et Zootechnica Sinica, 2026 , 57 (2) : 740 -752 . DOI: 10.11843/j.issn.0366-6964.2026.02.015
Appendix
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Year 2026 volume 57 Issue 2
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Article Info
doi: 10.11843/j.issn.0366-6964.2026.02.015
  • Receive Date：2025-07-04
  • Online Date：2026-09-16
  • Published：2026-02-23
Article Data
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History
  • Received：2025-07-04
Funding
Affiliations
    1.College of Animal Science and Technology，Ningxia University，Yinchuan 750021，China
    2.Ningxia Hui Autonomous Region Key Laboratory of Molecular Cell Breeding，Yinchuan 750021，China
    3.Ningxia Hui Autonomous Region Animal Husbandry Workstation，Yinchuan 750021，China
    4.College of Life Sciences and Engineering，Northwest Minzu University，Lanzhou 730030，China

Corresponding:

ZHANG Juan， E-mail：
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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