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Outline
The aim of this study was to understand the epidemic characteristics and drug resistance of Clostridium perfringens in Xizang’s Tibetan pig population， and to provide theoretical basis for disease prevention and control. In this study， 273 Tibetan pig fecal samples collected from 9 districts and counties in Xizang from 2021-2024 were isolated and identified by bacterial isolation and purification， PCR identification， drug sensitivity test， and drug resistance gene detection and other methods， and the toxin typing and drug resistance phenotype were determined. The results showed that a total of 180 strains of C. perfringens from Tibetan pigs were obtained， with an isolation rate of 65.93% （180/273）； According to toxin gene testing， all strains were identified as type A C. perfringens， with 142 strains containing the cpb2 gene. The results of antibiotic susceptibility tests showed that the isolated strains had high resistance to 6 drugs， including gentamicin， streptomycin， erythromycin， polymyxin B， sulfamethoxazole， trimethoprim. 97.22% （175/180） of the isolated bacteria were multidrug-resistant strains. The proportion of strains resistant to 4-6 types of antibiotics is 71.67% （129/180）. In addition， the isolated strains carried 12 resistance genes， with a detection rate of 23.08%（12/52）. The detection rate of the aminoglycoside resistance gene aadA was the highest， with a detection rate of 85.00%（153/180）. This study clarified the prevalence of C. perfringens from Tibetan pigs in Xizang， providing data support for taking effective prevention and control measures.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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