This article aims to elucidate how single-cell omics technologies advance livestock research and provides a systematic review of the construction process, current applications, and major challenges of single-cell transcriptomic databases in domestic animals. It seeks to offer data references and analytical frameworks for researchers, thereby promoting deeper application of this technology in livestock studies. This review conducted a comprehensive review and integrated analysis of existing single-cell transcriptomic database resources for livestock, summarizing their construction methodologies and application pathways, while also discussing current technical bottlenecks. The review demonstrates that single-cell transcriptomics offers novel perspectives and abundant data resources for elucidating mechanisms of livestock diseases, molecular breeding, and health management, while also revealing significant challenges in areas such as data integration and annotation standardization. Future efforts should focus on constructing high-quality databases, developing analysis algorithms tailored to livestock species, and promoting multi-omics integration to fully leverage the potential of single-cell technologies in the development of animal husbandry.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |