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The aim of this study was to understand the genomic characteristics and pathogenicity of epidemic porcine reproductive and respiratory syndrome virus （PRRSV） strains in China， and to explore their recombination and related variations. Pathological samples were collected， processed， and subjected to three consecutive blind passages in Marc-145 and PAM cells. Plaque purification was performed post-indirect IFA. Complete genomic sequences of the isolated strains were sequenced and analyzed. Piglet infection experiment was conducted for systematic assessment of the strains’ pathogenicity. In this study， six PRRSV type 2 strains were isolated and named WH-2021A， GZ-2021W， SX-2022M， HB-2021A， H-1， and C-1. WH-2021A， GZ-2021W， and SX-2022M belong to NADC30-like strains of L1.8.GZ-2021W is a recombinant strain with NADC30 as the main parent and highly pathogenic strain HuN4 as the secondary parent. SX-2022M is a recombinant strain with NADC30 as the main parent and classical strain CH-1a as the secondary parent. HB-2021A belongs to the L3.5 QYYZ branch， while H-1 and C-1 belong to the L8.7 highly pathogenic strain and the L5.1 classical strain， respectively. Piglets infection experiments showed that WH-2021A had the strongest pathogenicity， and the mortality rate of piglets was 100%， followed by H-1， with a mortality rate of 80%. The two recombinant strains of GZ-2021W and SX-2022M could cause a series of clinical lesions， but no piglets died. Pathological examination showed that the lesions of lung， lymph nodes and tonsils in the infected group were obvious. In this study， 6 epidemic PRRSV strains were isolated， and their genomic characteristics and pathogenicity were explored， which provides early warning of PRRS immune prevention， as well as critical data support for the recombinantion and evolutionary analysis of RRRSV-2.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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