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This experiment aims to study the immune-enhancing effects of peptide gCK-16 combined with the attenuated vaccine of infectious bursal disease （IBD） and to evaluate the protective effects of the vaccination on SPF chickens（14-day-old）. To investigate the toxicity of the peptide gCK-16， the immune effect of vaccine and the levels of cytokines （IFN-γ， IL-4 and IL-6） in serum， the cells were stimulated by the different concentrations of peptide gCK-16 in vitro， and then the chickens were immunized with the different concentrations of peptide gCK-6 in combination with attenuated vaccine of IBD. Meanwhile， the challenge protection of infectious bursal disease virus （IBDV） was performed by detecting the survival rate， the bursa-weight/body-weight index （BBIX）， the splenic lymphocyte viability， the level of immunoglobulin （IgM and IgA）， the level of immunoglobulin （IgY） in the serum， the histopathological changes and the expression level of apoptosis-related proteins in the bursa. The results show that peptide gCK-16 has no obvious toxicity both in vivo and in vitro， and peptide gCK-16 combined with the attenuated vaccine of IBD can significantly increase the antibody titer of IBDV in the serum of chickens immunized with the attenuated vaccine of IBD and the level of cytokines （IFN-γ， IL-4 and IL-6） in the serum. Moreover， the peptide gCK-16 significantly promoted the stimulation index of LPS， ConA and IBDV to PBMCs. Immunization and challenge experiments also showed that the peptide gCK-16 significantly increased the survival rate and BBIX of chickens infected with IBDV， and reduced the pathological changes of bursa tissues， while the expression levels of pro-apoptotic proteins Bax， Caspase-3， Caspase-9 were significantly decreased， and the expression level of anti-apoptotic protein Bcl-2 was significantly increased. In conclusion， peptide gCK-16 can significantly enhance the humoral and cellular immune responses of the attenuated vaccine of IBD， and achieved an effective immune protection in chickens infected with IBDV， which provides theoretical reference and data support for the application of this peptide as an immunopotentiator of the attenuated vaccine of IBD.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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