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Phage therapy is a novel antimicrobial treatment， in order to provide new theoretical and technical support for the prevention and treatment of E. coli infections， in this study， E. coli E6 and a broad-spectrum E. coli phage named vB_EcoM_E6 were isolated from the feces of diarrheic goats， and the potency， pH stability， thermal stability， lysis spectrum， and one-step growth curves of the phage were measured by observing the phage spots and biological properties， performing whole genome sequence analysis and evaluating the phage by animal model tests. The results showed that the lysis rate of this phage in the study was as high as 70.6%， and it was relatively stable at 50 ℃ and pH 3-10 range， and the transmission electron microscopy observation results indicated that this phage belonged to the myotail phage. Genome-wide analysis showed that the phage had a total length of 137 974 bp， a CG content of 39.13%， and 247 coding sequences （CDS）. The results of the mouse model test showed that the phage had a good protective effect against E. coli infection. This study provides a good material for further research on E. coli antimicrobial agents.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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