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This study aimed to prokaryotically express the lumpy skin disease virus （LSDV） ORF132 protein and evaluate its immunogenicity. The ORF132 gene was expressed in E. coli BL21（DE3）， purified， and emulsified with Freund's adjuvant. Mice were immunized with 100 µg of the protein per dose. The results demonstrated that the ORF132 protein elicited high titers of antigen-specific total IgG and a significantly elevated level of IFN-γ secretion compared to the PBS control group. Furthermore， it induced significant lymphocyte proliferation and a high neutralizing antibody titer. These findings indicate that the ORF132 protein is an immunodominant antigen capable of inducing robust humoral and cellular immune responses in mice， providing a promising candidate for subsequent multi-antigen combination studies and bovine trials.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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