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The aim was to study the effects of the ethanol extract of chicory （CIL）， a medicinal and edible homology herb， on the hepatic lipid metabolites of non-alcoholic fatty liver disease （NAFLD） induced by high-fat diet （HFD）， and to analyze the mechanism of chicory action in ameliorating the disorders of lipid metabolism. HFD was induced to establish a NAFLD model in C57BL/6J mice， and the therapeutic effects of CIL （800 mg·kg-1 and 2 g·kg-1） and simvastatin （SVT， 20 mg·kg-1） were evaluated in NAFLD mice. Measurement of liver aspartate aminotransferase （AST） and alanine aminotransferase （ALT） enzyme activity， catalase （CAT） activity， malondialdehyde （MDA） content， and tumor necrosis factor-α （TNF-α） and interleukin-10 （IL-10） content. The composition of hepatic lipid metabolites was detected using UPLC-MS/MS lipidomics； qRT-PCR was used to detect the expression levels of mRNA genes for lipid synthesis-related genes （PPARγ， ACCα， Srebp1c and CD36） and lipid oxidation-related genes （PPARα and CPT1α）. The results of in vivo experiments showed that high-dose chicory alcohol extract group （HCIL） suppressed HFD-induced abnormal elevation of AST and ALT highly significantly （P<0.01）， and there was no significant difference compared with the SVT group enzyme activity （P>0.05）. The CIL treatment highly significantly reduced blood and liver triglyceride （TG） levels （P<0.01）， and there was no significant difference in TG levels with the SVT group （P>0.05）. The HCIL treatment obviously alleviated hepatic steatosis and fat cell volume； remodeled the lipid composition of liver， lowered hepatic TG and DG levels， and elevated the levels of PG， PE， LNAPE， LPE， and HexCer. Both the HCIL group and the SVT group highly significantly lowered the hepatic expression levels of PPARγ， ACCα， Srebp1c， and CD36 mRNA genes （P<0.01）， targeted inhibition of lipid uptake by CD36 protein， and molecular docking with high binding energy. Therefore， CIL regulates lipid metabolism disorders by targeted inhibition of CD36 lipid uptake， improving liver lipid composition. The pharmacological effects of CIL treatment for NAFLD are not significantly different from those of SVT drug treatment， which will promote the development and application of chicory products in the livestock and poultry farming industry.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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