Full
Outline
Rabbit coccidiosis is a worldwide-distributed parasitic protozoal disease， which has caused huge economic losses to rabbit industry. Traditional methods of anticoccidial treatment result in drug resistance and drug residue， vaccination is a promising alternative. The rEiAMA1， rEiIMP1， EmGAM56， and EmROP17 were respectively displayed on the surface of B. subtilis spores by using a coat protein （CotB） as an anchor. Then the recombinant spores were used to oral immunize rabbits， the protective effects of B. subtilis-rEiAMA1， -rEiIMP1， and -rEiAMA1+rEiIMP1 against Eimeria intestinalis were evaluated； and the protective effects of B. subtilis-EmGAM56， -EmROP17， and -EmGAM56+ EmROP17 against Eimeria magna were evaluated. The results showed that B. subtilis-rEiAMA1 immunized rabbits displayed better anti-E. intestinalis protective effect， the oocyst output and lesion score were significantly decreased （P<0.05）， the oocyst output decrease ratio was 59.91%. B. subtilis-EmGAM56 immunized rabbits displayed better anti-E. magna protective effect， the oocyst output was significantly decreased （P<0.05）， the oocyst output decrease ratio was 68.54%. Moreover， the immunized rabbits showed significantly increased specific serum IgG levels and specific IgA levels （P<0.05）. The four recombinant B. subtilis spores can stimulate the body to produce specific intestinal and systemic humoral immune responses. B. subtilis-rEiAMA1 and B. subtilis-EmGAM56 showed better immunoprotection against E. intestinalis and E. magna， respectively. Therefore， B. subtilis is a potential live vector for rabbit coccidiosis vaccines.
152
82
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
