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Effects of Pediococcus pentosaceus 368 on Fecal Archaea and Fecal Metabolites of Duroc×Saba Pig
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Shaona LIU1, Yan ZHANG1, Heli XIONG1, Zhixiang ZHANG1, 2, Wenjing WAN1, 2, Zhiyong ZHAO1
Acta Veterinaria et Zootechnica Sinica | 2026, 57(2) : 848 - 860
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Acta Veterinaria et Zootechnica Sinica | 2026, 57(2): 848-860
• ANIMAL NUTRITION AND FEEDS •
Effects of Pediococcus pentosaceus 368 on Fecal Archaea and Fecal Metabolites of Duroc×Saba Pig
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Shaona LIU1, Yan ZHANG1, Heli XIONG1, Zhixiang ZHANG1, 2, Wenjing WAN1, 2, Zhiyong ZHAO1
Affiliations
  • 1.Yunnan Animal Science and Veterinary Institute,Kunming 650224,China
  • 2.Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China
Published: 2026-02-23 doi: 10.11843/j.issn.0366-6964.2026.02.024
Outline
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The study aimed to explore the effects of Pediococcus pentosaceus 368 on Archaea and metabolites in feces of Duroc×Saba pig. Twenty Duroc×Saba pigs which body weight were about 77 kg were randomly divided into 2 groups with 10 pigs per group. The pigs in experimental group drinked water added with Pediococcus pentosaceus 368(1010 CFU·L-1). The experiment lasted for 28 days. On the day at the end of the experiment, slaughterings were conducted. The effects of Pediococcus pentosaceus 368 on Duroc×Saba pig were analyzed through the Archaea in feces and the fecal metabolites. The results showed as follows: 1) PCoA (Principal Coordinates Analysis) analysis found that samples in the experimental group and control group could not be completely separated, and species similarity in the experimental group became consistent after the addition of Pediococcus pentosus 368.2) At the phylum level, the highest relative abundance of the two groups was the Candidatus_Thorarchaeota and at the genus level, the highest relative abundance of the two groups was Methanobrevibacter. When the Linear Discriminant Analysis value was highter than 3.0, the two groups obtained 14 biomarkers. Among them, 14 biomarkers were in the experimental group, and the largest LDA value in the experimental group was the Thermoplasmata_unclassified.3) KEEG metabolic pathway analysis found that when the LDA value was highter than 3.0, the functions of 4 genes were screened out: Folding_sorting_and_degradation, membrane transport,Cell motility and Signal transduction; Analysis of the level function of CAZy (Carbohydrate Active enzyme Database) showed that when the LDA value was highter than 3.0, a total of 21 biomarkers were obtained in the two groups, of which 13 were in the experimental group. The biomarker with the largest LDA value in the experimental group was CBM20. In the control group, the biomarker with the largest LDA value was GT2.4) Fecal short-chain fatty acid analysis showed that the contents of Butyric acid and Isovaleric acid in the experimental group were significantly decreased (P<0.05). 5) KEGG pathway enrichment analysis of differential metabolites found that there were 12 significantly different terms, which were as follows: Degradation of flavonoids,Linoleic acid metabolism, Cutin, suberine and wax biosynthesis, Arginine biosynthesis, Valine, leucine and isoleucine biosynthesis, Retrograde endocannabinoid signaling, Biosynthesis of phenylpropanoids,Biosynthesis of unsaturated fatty acids, Autophagy-animal, Fatty acid biosynthesis,Kaposi sarcoma-associated herpesvirus infection; Gene Set Enrichment Analysis (GSEA) identified three significant genes: Glycerophosphates metabolism; Glycine, serine and threonine metabolism; and Biosynthesis of unsaturated fatty acids. (Conclusion) In summary, the addition of Pediococcus pentosaceus 368 can make the species similarity become consistent in the fecal Archaea of Duroc×Saba pigs. Improve the relative abundance of microbial functional genes related to Signal transduction, Cell motility, amino acid and fat metabolism in feces, and improve the probiotic function of bacteria and enzymes related to amino acid and fat metabolism.

Duroc×Saba pig  /  Pediococcus pentosaceus  /  Archaea  /  LC-MS
刘韶娜, 张彦, 熊和丽, 张志翔, 万文璟, 赵智勇. 戊糖片球菌368对杜撒猪粪便中古菌及代谢产物的影响. 畜牧兽医学报, 2026 , 57 (2) : 848 -860 . DOI: 10.11843/j.issn.0366-6964.2026.02.024
Shaona LIU, Yan ZHANG, Heli XIONG, Zhixiang ZHANG, Wenjing WAN, Zhiyong ZHAO. Effects of Pediococcus pentosaceus 368 on Fecal Archaea and Fecal Metabolites of Duroc×Saba Pig[J]. Acta Veterinaria et Zootechnica Sinica, 2026 , 57 (2) : 848 -860 . DOI: 10.11843/j.issn.0366-6964.2026.02.024
Year 2026 volume 57 Issue 2
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doi: 10.11843/j.issn.0366-6964.2026.02.024
  • Receive Date:2025-01-06
  • Online Date:2026-09-16
  • Published:2026-02-23
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  • Received:2025-01-06
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Affiliations
    1.Yunnan Animal Science and Veterinary Institute,Kunming 650224,China
    2.Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China

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表12种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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