Full
Outline
The formation of green eggshell characteristics in chickens is associated with the high expression of Solute Carrier Organic Anion Transporter Family Member 1B3 （SLCO1B3） in eggshell glands initiated by the endogenous retrovirus EAV-HP. As a core metabolic organ， SLCO1B3 is highly expressed in the chicken liver. The insertion mutation of EAV-HP downregulates the expression of SLCO1B3 in the chicken liver. Metabolomics studies of chicken liver during this process have not been reported. This study took the Luhua chicken as the research object. Using the green-shelled egg genotypic primers， it was determined that the frequency of green-shelled eggs produced in 38 Luhua chickens was 47.4%. Both the integration of EAV-HP+/+ and EAV-HP+/- significantly reduced the transcriptional level of SLCO1B3 in the liver of Luhua chicken （P<0.05）， especially the effect of the integration of EAV-HP+/+ was especially significant. A total of 80 differential metabolites were screened out by metabolomics， among which 30 were up-regulated and 50 were down-regulated. The pathways through which metabolites are enriched mainly involve biological processes such as taurine and hypotaurine metabolism， the citric acid cycle （TCA cycle）， carbohydrate metabolism， ether lipid metabolism and alanine， aspartic acid and glutamic acid metabolism. The research results revealed the effect of down-regulation of SLCO1B3 expression caused by EAV-HP insertion mutation on liver metabolites and metabolic pathways in chickens， providing research ideas and directions for fully understanding and utilizing endogenous retroviruses to improve the production performance of chicken flocks.
122
62
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
