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The purpose of this study was to understand the prevalence and drug resistance characteristics of Klebsiella pneumoniae in dairy cow mastitis in Xinjiang. Two thousand and eighty-three milk samples of dairy cows with clinical mastitis or subclinical mastitis were collected from 15 large-scale dairy farms in 9 areas of intensive dairy farming in Xinjiang. Klebsiella pneumoniae in the samples was isolated and cultured， Gram stained. PCR and 16S rRNA sequencing were conducted to identified the isolated strains， and the drug resistance of the isolated strains was detected by micro-broth dilution method. The results showed that 266 strains of Klebsiella pneumoniae were isolated from the milk samples， and the isolation rate was 12.8% （266/2 083）.There was no significant difference in the isolation rate of Klebsiella pneumoniae between clinical mastitis and subclinical mastitis （P>0.05）. The sensitivity of 266 isolates to 16 antimicrobial agents and the production of extended-spectrum β-lactamases （ESBLs） were determined by broth microdilution method. The results showed that the resistance rates of the isolates to tetracycline， ceftiofur， cefepime， cefotaxime and cefazolin were high （23.7%-41.0%）. And 16.9% （45/266） of the isolates were multidrug-resistant bacteria， and 6.4% （17/266） of the isolates produced ESBLs. The results suggest that the average separation rate of mastitis Klebsiella pneumoniae in 15 large-scale dairy farms in Xinjiang is at a relatively low level in the whole country. The isolated strains had strong resistance to tetracycline and cephalosporin antibiotics. This study provided a basis for mastering the epidemiological characteristics of Klebsiella pneumoniae in dairy cow mastitis and guiding clinical medication.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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