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The objective of this experiment was to investigate the effects of different calcium （Ca）， non-phytic phosphorus （NPP） levels and electrolyte balance （DEB） value in a low protein diet on growth performance， serum biochemical indices and metabolic rate of nutrients in broilers. A 3×3 two-factor completely randomized design was adopted. Three dietary calcium and non-phytate phosphorus levels were 0.70% Ca and 0.35% NPP， 0.80% Ca and 0.40% NPP， 0.90% Ca and 0.45%NPP， respectively. And three levels of dietary electrolyte balance （Na++K+-Cl-） were 200， 250 and 300 mmol·kg-1， respectively. In addition， dietary crude protein level was 19.5%. A total of 324 1-day-old Arbor Acres （AA） male broilers were randomly allotted to 1 of 9 treatments with 6 replicate cages per treatment and 6 birds per cage. The experimental period was 21 days. The results showed that： 1） Dietary Ca， NPP and DEB value and their interaction had no significant effect （P>0.05） on growth performance of broilers from 1 to 21 d of age； 2） The interaction of dietary Ca， NPP and DEB value had significant effect （P<0.05） on the alanine aminotransferase （ALT） activity in serum. As dietary Ca and NPP levels were 0.70% and 0.35%， the serum ALT activity from broilers in the 300 mmol·kg-1 DEB value group was significantly higher （P<0.05） than that of the 200 and 250 mmol·kg-1 DEB value groups. As the levels of Ca and NPP were 0.80% and 0.40%， the serum ALT activity from broilers in the 250 or 300 mmol·kg-1 DEB value groups was significantly lower （P<0.05） than that in the 200 mmol·kg-1 DEB value group. As the levels of Ca and NPP were 0.90% and 0.45%， DEB levels had no significant effect （P>0.05） on serum ALT activity； 3） As dietary Ca and NPP levels increased， the apparent metabolic rate of Ca decreased significantly （P<0.05）. The apparent metabolic rates of dry matter， crude protein and Ca in broilers from 300 mmol·kg-1 DEB value group were significantly higher （P<0.05） than those in 250 and 200 mmol·kg-1 DEB value groups. As the levels of dietary Ca and NPP were 0.70% and 0.35%， 300 mmol·kg-1 DEB value could significantly increase （P<0.05） the apparent metabolic rate of P compared with the 200 and 250 mmol·kg-1 DEB value. And as the levels of Ca and NPP were 0.90% and 0.45%， the apparent metabolic rate of P in 300 mmol·kg-1 DEB value group was significantly higher （P<0.05） than that in the 200 mmol·kg-1 group. These results indicated that the Ca and NPP levels of 0.80% and 0.40% and DEB value of 300 mmol·kg-1 in a low protein diet （Crude protein： 19.5%） were optimal for the growth and nutrient utilization in broilers from 1 to 21 d of age.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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