Full
Outline
This study aimed to analyze the metabolic mechanisms of testicular tissue associated with semen quality in ducks from a metabolomics perspective. Semen quality was evaluated in a duck population. Twelve drakes （6 with significantly high semen quality and 6 with significantly low semen quality） were selected， and testicular tissues were collected. Ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） was used to analyze differences in testicular metabolites between groups. Enrichment analysis of differential metabolites was performed to screen potential key pathways related to testicular semen quality. A total of 98 differentially abundant metabolites was identified in testicular tissues of ducks with varying semen quality， including 15 significantly upregulated and 83 significantly downregulated metabolites. These metabolites primarily belonged to categories such as fatty acyls， amino acids and their metabolites， glycerophospholipids， nucleotides and their metabolites， benzene derivatives， coenzymes and vitamins， and hormones. Correlation analysis revealed the strongest positive correlation between fatty acyls and benzene derivatives， while the strongest negative correlation was observed between carbohydrates/their metabolites and aldehydes/ketones/esters. Semen quality parameters （e.g.， sperm motility and kinematic parameters） showed significant positive/negative correlations （|R|≥0.80） with fatty acyls， glycerophospholipids， and other metabolites. Enrichment analysis highlighted two critical metabolic pathways： fatty acid degradation （P=0.000 59） and glycerophospholipid metabolism （P=0.012 76）. This study elucidated differential metabolites and potential metabolic mechanisms associated with semen quality in duck testicular tissues through metabolomics. It identifies 98 differential metabolites dominated by lipids （fatty acyls and glycerophospholipids）， characterized by significant downregulation of carnitines and enrichment of hexadecanoic acid （FFA16：0） and maltitol in the high semen quality group， and identifies fatty acid degradation and glycerophospholipid metabolism as core pathways significantly associated with semen quality. These findings provide key metabolites and pathway targets for research on avian testicular function.
142
68
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
