This study aimed to investigate the differences in fur quality and the potential molecular mechanisms influencing the coarse hair percentage of hybrid offspring of Rex rabbits and Hycole meat rabbits， in order to provide a reference for research on fur quality of hybrid rabbits. Twelve 165-day-old F2-generation rabbits produced by crossing Rex rabbits and Hycole meat rabbits were selected. Fur quality indicators， including hair length， hair fineness， and coarse hair percentage， were measured. Based on the statistical result of coarse hair percentage， 10 female rabbits were further selected for transcriptomic sequencing of skin tissues. Among them， 5 individuals with coarse hair percentage ≥ 10% were classified as the high coarse hair percentage group， and 5 with coarse hair percentage <10% were classified as the low coarse hair percentage group. Differentially expressed genes （DEGs） between the two groups were compared， followed by functional enrichment analysis and protein-protein interaction （PPI） network construction. The accuracy of the sequencing results was verified using quantitative real-time PCR. The results showed that in the F2 hybrid rabbits， coarse hair percentage was extremely significantly positively correlated with hair length （P<0.01） and significantly positively correlated with hair fineness （P<0.05）， but not significantly correlated with other indicators （P>0.05）. A total of 281 DEGs were identified between the high and low coarse hair percentage groups. Gene Ontology （GO） enrichment analysis revealed these genes were significantly enriched in functional terms such as drug transport， regulation of myoblast proliferation， contractile fiber， and glycosaminoglycan binding. Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analysis showed significant enrichment in important biological processes including glycolysis/gluconeogenesis， steroid hormone biosynthesis， motor proteins， and the PPAR signaling pathway. A protein-protein interaction （PPI） network of the differentially expressed proteins was constructed， and 4 candidate genes were identified （FGF7， MMP3， MMP13 and FOXP3）. RT-qPCR results confirmed that the expression trends of these 5 DEGs were largely consistent with the RNA-seq data. These results suggest that the FGF7， MMP3， MMP13 and FOXP3 genes may be important candidate genes influencing the coarse hair percentage trait. This study provides data support for evaluating the fur quality of hybrid offspring between Rex and Hycole meat rabbits and offers insights for improving fur traits in hybrid breeds.