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Study on the Synthesis of Floridoside Through Trans-Glycosylation by α-Galactosidase rAgaAJB07
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Xi SHEN1, 4, Meng TIAN1, Yue-lu ZHU1, Xin-yue LI1, Ling-xi QI1, Rui ZHANG1, 2, 3, 4, Jun-pei ZHOU1, 2, 3, 4, *
Journal of Microbiology | 2026, 46(2) : 36 - 43
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Journal of Microbiology | 2026, 46(2): 36-43
Study on the Synthesis of Floridoside Through Trans-Glycosylation by α-Galactosidase rAgaAJB07
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Xi SHEN1, 4, Meng TIAN1, Yue-lu ZHU1, Xin-yue LI1, Ling-xi QI1, Rui ZHANG1, 2, 3, 4, Jun-pei ZHOU1, 2, 3, 4, *
Affiliations
  • 1.School of Life Sciences, Yunnan Normal University, Kunming 650500
  • 2.Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan Normal University, Kunming 650500
  • 3.Key Laboratory of Yunnan Provincial Education Department for Plateau Characteristic Food Enzymes, Yunnan Normal University, Kunming 650500
  • 4.Southwest United Graduate School, Kunming 650092
Published: 2026-04-20 doi: 10.3969/j.issn.1005-7021.2026.02.004
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Floridoside, a glycosyl alcohol exhibiting diverse biological activities including antioxidant effects, immune regulation, and protein stabilization, demonstrates significant potential for applications in the food and cosmetic industries. In our previous study, we successfully achieved the heterologous expression of the recombinant α-galactosidase rAgaAJB07 from Mesorhizobium, which catalyzes floridoside biosynthesis via trans-glycosylation was obtained. In order to improve floridoside production, the reaction conditions were systematically optimized for the recombinant enzyme using glycerol as the acceptor substrate and p-nitrophenyl α-D-galactopyranoside (pNPG) as the donor substrate. The optimal parameters were determined as follows: 500 mmol/L glycerol, 40 mmol/L pNPG, 1.5 U/mL enzyme concentration, 2 h reaction duration, pH 8.5, and 30 ℃. Under these conditions, recombinant rAgaAJB07 achieved a maximum floridoside yield with a 66.2% enhancement compared to baseline parameters, while simultaneously reducing the reaction time by 22 hours. This optimization strategy provides a robust methodological framework for scalable enzymatic synthesis of floridoside.

glucoside hydrolase  /  α-galactosidase  /  trans-glycosylation synthesis  /  floridoside  /  yield optimization
申熙, 田萌, 朱悦露, 李新月, 祁玲曦, 张蕊, 周峻沛. α-半乳糖苷酶rAgaAJB07转糖基合成红藻糖苷的研究. 微生物学杂志, 2026 , 46 (2) : 36 -43 . DOI: 10.3969/j.issn.1005-7021.2026.02.004
Xi SHEN, Meng TIAN, Yue-lu ZHU, Xin-yue LI, Ling-xi QI, Rui ZHANG, Jun-pei ZHOU. Study on the Synthesis of Floridoside Through Trans-Glycosylation by α-Galactosidase rAgaAJB07[J]. Journal of Microbiology, 2026 , 46 (2) : 36 -43 . DOI: 10.3969/j.issn.1005-7021.2026.02.004
Appendix
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Year 2026 volume 46 Issue 2
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Article Info
doi: 10.3969/j.issn.1005-7021.2026.02.004
  • Receive Date：2025-07-07
  • Online Date：2026-10-10
  • Published：2026-04-20
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History
  • Received：2025-07-07
Funding
Affiliations
    1.School of Life Sciences, Yunnan Normal University, Kunming 650500
    2.Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan Normal University, Kunming 650500
    3.Key Laboratory of Yunnan Provincial Education Department for Plateau Characteristic Food Enzymes, Yunnan Normal University, Kunming 650500
    4.Southwest United Graduate School, Kunming 650092
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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